摘要
本文采用植物组织培养方法,探讨内蒙野丁香不同组织器官在离体培养条件下被诱导形成愈伤组织,及进一步分化形成再生植株的过程和条件,结果表明:不同外植体的诱导率是茎>叶>根。激素种类及其浓度是器官脱分化与植株再生的决定因素,诱导愈伤组织的最适培养基为MS+2mg/L 6-BA+10mg/L IAA;芽分化诱导的最适培养基为MS+0.6mg/L 6-BA+5mg/L IAA;生根的最适培养基为MS+0.01mg/L NAA。
The process and condition in which Leptodermis ordosica＇s different tissue organs were induced into calluses and recover regeneration regenerated plant were studied. The results showed that the inducing efficiency of different explants are stems 〉 leaves 〉 roots. The kinds and concentration of hormone are the decisive factor of organ dedifferentiation and redifferentiation, and the optimum culture medium of inducing calluses is MS ＋ 2mg/L 6 - BA ＋ 10mg/L IAA ; the optimum culture medium of inducing bud is MS ＋ 1 mg/ L 6 - BA ＋5mg/L IAA and the optimum culture medium of rooting is MS ＋0.05mg/L NAA.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2007年第1期25-30,共6页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
关键词
珍稀濒危植物
内蒙野丁香
组织培养
Rare plant
Leptodermis ordosica H. C. Fu et E. W. Ma
tissue culture
