摘要
对番茄花叶病毒番茄分离物（ＴｏＭＶ－Ｔｏ－Ｓ１）和烟草花叶病毒蚕豆分离物（ＴＭＶ－Ｂ９３５Ａ）进行了生物学和血清学比较。人工接种６科２０种植物，这两个分离物均能侵染其中的３科１４种植物，但在普通烟（白肋烟、黄苗榆）上的症状差异较大，前者在这些植物上引起局部症状，后者为系统症状。在琼脂双扩散试验中，两者与ＴｏＭＶ－Ｔｏ－Ｓ１抗血清和４种ＴＭＶ抗血清均形成沉淀线，但沉淀线之间产生刺角，说明ＴｏＭＶ－Ｔｏ－Ｓ１和ＴＭＶ－Ｂ９３５Ａ虽有血清关系，但亦有差异。胶内交叉吸附试验也证明两者之间有血清学差异。根据已报道的ＴｏＭＶ－Ｌ株系和ＴＭＶＵ１株系ＣＰ基因的３′端非编码区序列设计了ＴｏＭＶ和ＴＭＶ特异引物，采用ＰＣＲ方法，这些引物能够特异性的检测区分ＴｏＭＶ－Ｔｏ－Ｓ１和ＴＭＶ－Ｂ９３５Ａ。
Biology and serological comparison between ToMV tomato isolate (ToMV To S1) and TMV broad bean isolate (TMV B935A) was made. Both ToMV To S1 and TMV B935A could infected 14 plant species in 3 families when 20 plant species in 6 families were tested. The difference was found in Nicotiana tabacum ( N. tabacum White Burley, N. tabacum Huang Miaoyu), ToMV To S1 induced local lesions in these plant species, while TMV B935A produced systemic mosaic symptoms. In double diffusion tests, spur formation was observed between ToMV To S1 and TMV B935A, and the results of intragel cross absorption tests agreed with the doublediffusion tests results. Specific primers corresponding to the coat protein gene and 3′ non coding region were designed. When ToMV To S1 cDNA templete were used, a specific band could be amplified with ToMV primers by PCR, No band was found when we used TMV primers. With TMV B935A templete, only TMV primers could produce a specific band. So it is easy to distinguish ToMV To S1 and TMV B935A with ToMV and TMV specific primers.
出处
《植物病理学报》
CAS
CSCD
北大核心
1997年第1期53-58,共6页
Acta Phytopathologica Sinica
