摘要
目的探讨光动力疗法(PDT)对宫颈恶性肿瘤杀伤作用的机制。方法应用PDT对接种Me180宫颈癌细胞系的12只裸鼠做肿瘤杀伤的对比实验研究。通过实时荧光PCR对Survivin基因的相对定量分析,以明确基因表达水平的变化。并采用免疫组化SP法对凋亡抑制基因Survivin蛋白表达水平进行检测。结果通过Survivin基因和蛋白表达的检测证实,治疗组mRNA及蛋白的表达较对照组显著降低(P<0.01),PDT所致的细胞死亡与Survivin途径密切相关。结论PDT治疗后Survivin基因和蛋白的表达均明显降低,Survivin可望成为评价PDT疗效的分子指标之一。
Objective：To investigate the mechanism of the killing effect of photodynamic therapy （PDT）on cervical cancer.Methods：The killing experiment via PDT was carried out in 16 nude mice inoculated with Mel80 cervical cancer.The expression of surviving was detected by immunohistochemical stain and the relative quantification in real-time RT-PCR.Results：Survivin expression in the treatment group wais dramatically lower than that in the control group（P〈0.01）.The pathway of cell apoptosis was correlated with survivin.Conclusion： The decrease of survivin expression after PDT suggested that it may be used to predict the effect of PDT.
出处
《中日友好医院学报》
2007年第3期159-161,F0004,共4页
Journal of China-Japan Friendship Hospital
基金
中国医学科学院院级课题(20053064)资助。
关键词
光动力疗法
宫颈癌
免疫组化
实时荧光聚合酶链反应
裸鼠
photodynamic therapy
cervical cancer
immunohistochemistry
real time polymerase chain reaction
nude mice
