摘要
目的观察兔全脑缺血再灌注（I／R）期间颈内静脉血浆ET、NO的动态变化及它们与脑I／R损伤的关系。方法取清洁级家兔24只，随机分A、B、C3组，每组8只，缺血15min后分别再灌注30min（A组），2h（B组）和4h（C组），C组在缺血前15min（L0）及再灌注30min（R1）、2h（R2）、4h（R3）4个时点、B组在前3个时点、A组在前2个时点采颈内静脉球部血样分别测定ET、N0及计算ET／N0比值；取左颞皮质作HE、尼氏体染色，光镜观察，对神经元进行图像分析，测量核截面积和尼氏体平均光密度。结果（1）与L1比较，NO在R1时显著增加（P〈0．01），在＆、飓时缓慢下降；ET在R1～R2时持续上升，由317．58ng／L升至637．01ng／L；在R1时ET／NO比值与L0比较差异无显著性，在R2、R3时比值显著增大，由44．83×10^-6升至224．30X10^-6；（2）B、C组的病理损伤比A组严重，C组又比B组严重；B、C组的神经元核截面积比A组显著降低，C组的核截面积最小；尼氏体平均光密度A〉B〉C组；（3）ET分别与核截面积及尼氏体平均光密度呈显著负相关；ET／N0比值与核截面积及尼氏体平均光密度呈非常显著负相关；结论ET增加及ET与NO之间平衡的破坏可能是脑I／R损伤的主要原因之一；检测颈内静脉血ET和NO的动态变化可以反映脑I／R损伤程度。
Objective To observe the dynamic changes of endothelin （ET） and nitric oxide（NO） concentration of internal jugular venous blood plasm in rabbits with global cerebral ischemia/reperfusion（I/R） and the relationship between ET, NO changes and cerebral I/R injury. Methods Twenty-four rabbits were randomly divided into A, B, C groups. Internal jugular venous blood samples were taken at 15 min before ischemia（L） and 30 min （R1）, 2 h（R2 ）, and 4 h（R3 ） after reperfusion in C group, at L0, R1 and R2 in B group and at L0, R1 in A group for detecting the changes of concentrations of ET, NO and ET/NO. The left temporal cortex was harvested and followed by hematoxylin and eosin （HE） and Nissl dyeing to observe the morphological changes of neurons under light microscopy. Cross-sectional area and Nissl average optical density（AOD） of nu- clei were measured by image analysis system. Results （1） The plasma concentration of NO was significantly increased at R1 （P〈0. 01 ） and slowly decreased at R2 and R3. The ET concentration was persistently improved during R1-R3 from 317. 58 ng/L to 637. 01 ng/L （2） ET/NO had no significant difference between R1 and L, but it increased obviously from 44. 83 × 10^-6 to 224. 30×10^-6 at R2 and R3. （3） The pathological damages of B and C group were more serious than that of A group while C group was more serious than B group. （4） The nucleus cross-sectional area of B and C group was significantly decreased compared to A group and it was least in C group. The trend of Nissl AOD was A〉B〉C group. （5）ET was negatively correlated with nucleus cross-sectional area and Nissl AOD, while ET/NO was positively correlated with them. Conclusions （1） The increase of ET was the main reason of cerebral I/R injury. The relationship is closer between ET/NO unbalanceand cerebral I/R injury. （2） The dynamic tests of ET and NO concentration in internal jugular venous play an important role in evaluation the degree and outcome of cerebral I/R injury.
出处
《卒中与神经疾病》
2007年第3期138-140,153,共4页
Stroke and Nervous Diseases
关键词
内皮素
一氧化氮
脑再灌注损伤
颈内静脉血
尼氏体
核截面积
Endothelin Nitric oxide Cerebral ischemia/reperfusion Internal jugular venous bloodNissl body Nucleus cross-sectional area
