摘要
目的建立通脉降脂颗粒剂的质量标准。方法采用薄层层析(TLC)法对通脉降脂颗粒中的姜黄素、大黄酸、大黄酚、大黄素、芦荟大黄素进行定性鉴别,高效液相色谱(HPLC)法定量测定姜黄素、大黄酸、大黄酚、大黄素的含量。结果用C18柱,流动相为甲醇-水-乙酸(77∶22∶1),检测波长为428nm,测定结果的线性关系良好。姜黄素、大黄酸、大黄酚、大黄素的平均回收率分别为97.75%,96.65%,97.79%,97.17%。结论采用高效液相色谱法对通脉降脂颗粒进行质量控制,方法简便,准确,重复性好。
Objective The quality standard of Tongmai Jiangzhi Granule has been established. Methods TLC was used to identify curcumin, rhein, emodin, chrysophanol, aloe - emodin. HPLC was used to determine the contents of cureumin, rhein, acidin, and chrysophanol. Results The linear relation was excellent for determination with C18 column methanol - water - acetic acid （77：22：1 ） as mobile phase. The average recoveries of curcumln, rhein, emodin, chrysophanol were 97.75% ,96.65% , 97.79% and 97.17% , respectively. Conclusion The methods are available with a good reproducibility and can be used to control the quality of Tongmai Jiangzhi Granule.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第3期634-635,共2页
Lishizhen Medicine and Materia Medica Research
