摘要
该研究应用ELISA双抗夹心法对尿路感染（UTI）患者血清和尿sIL－2R、IL－8水平进行了测定。结果表明：UTI患者尿IL－8浓度与尿白细胞数量呈显著正相关（n＝46r’s＝0．401，P＜0．01）；肾工肾炎患者尿IL－8浓度显著高于血清浓度（P＜0.05），提示尿IL－8主要由肾脏局部产生且可能是中性粒细胞自血流游入尿中的重要因素之一；膀胱炎患者血清和尿sIL－2R水平显著低于健康人（P＜0．05），提示T细胞功能低下可能是UTI的易感因素之一；急性肾孟肾炎（APN）患者血清和尿IL－8水平显著高于其它各组（P＜0．001）．血清和尿sIL－2R水平显著高于对照组（P＜0．05），提示中性粒细胞、T细胞在APN机体防御中起重要作用，同时也造成肾损伤；慢性肾盂肾炎（CPN）患者血清和尿sIL－2R水平显著高于健康人（P＜0.01．P＜0．001），尿IL－8水平显著高于健康人（P＜0，02）且与尿sIL－2R浓度显著正相关（n＝18，r＝0.536，P＜0．05），提示在CPN中T细胞可能是参与机体防御的重要细胞，局部免疫功能紊乱可能是炎症持续进展的原因之一；血清和尿sIL－2R、IL－8检测对上、下UTI的鉴别有参考价值。
Serum and urine levels of sIL - 2R and IL - 8 Were determined by using sandwich ELISA in patients with urinary tract infections (UTI). There was a positive correlation between the IL - 8 Ievels and the semiquantitativecounts of polymorphonuclear leukocytes (PMN) in urine (n = 46, r = 0. 401, P < 0. 01). In the patients withpyelonephritis, urine IL - 8 levels were significantly higher than serum IL - 8 levels (P< 0. 05 ), suggesting thaturine IL - 8 is produced in the kidney and is responsible for the transmigration of neutrophils from the bloodstreamto the urinary tract lumen. In the patients with cystitis, serum and urine sIL - 2R Ievels were significantly lowerthan thase in healthy controls (P< 0. 05 ). The evidence suggests that impaired T-lymphocyte function is a susceptible factor of UTI. In the patients with acute pyelonephritis (APN), serum and urine IL - 8 levels were significantlyhigher than those in the other groups (P< 0. 001). Serum and urine sIL- 2R levels were significantly higher thanthose in the healthy controls (P< 0. 05), suggesting that neutrophils and T-lymphocytes play important roles in hostdefences and result in renal damage in APN. In patients with chronic pyelonephritis (CPN), serum and urine sIL-2R levels were significantly higher than those in healthy controls (P< 0.02) and there was a positive correlation between the IL - 8 levels and the sIL - 2R levels in urine (n = 18, r = 0. 544, P< 0. 02). These findings suggest thatT-lymphocytes are important cells in host defences and that localized immune dysfunction is one of the causes of continuity and progress of inflammation in CPN. The 1evel of sIL - 2R and IL - 8 in urine and serum will contribute todistinguishing an upper UTI from a lower UTI.
出处
《中国现代医学杂志》
CAS
CSCD
1997年第2期8-11,共4页
China Journal of Modern Medicine
关键词
膀胱炎
急性
肾盂肾炎
慢性
白细胞介素-8
Cystitis
Acute pyelonephritis
Chronic pyelonephritis
sIL-2R
IL-8
