摘要
目的观察经密达利(40%四聚乙醛水剂)处理的钉螺组织酶活性变化,探讨密达利杀螺机制。方法取湖北钉螺60只,均分2组。实验组用100mg/L密达利浸泡2d,取存活的钉螺,分离完整的软体组织,制作冷冻切片,用酶组织化学方法对乳酸脱氢酶(LDH),琥珀酸脱氢酶(SDH)、细胞色素C氧化酶(CCO)、乙酰胆碱酯酶(AChE)和一氧化氮合酶(NOS)进行染色,用显微图像分析系统定量测定其平均灰度值,每种酶均取10只钉螺组织切片测定其灰度值。同时设未经药物处理的湖北钉螺对照组。结果钉螺肝组织、口囊组织的CCO灰度值,实验组分别为0.2042±0.0463和0.1695±0.0526,分别低于对照组的0.5556±0.0878和0.6082±0.0723,两者差异有统计学意义(t=12.26,P<0.01);神经节的AChE灰度值,实验组为0.2618±0.0330,低于对照组的0.5100±0.0295,差异有统计学意义(t=18.72,P<0.01)。口囊、肌纤维中的LDH灰度值,实验组分别为0.5801±0.0982和0.4043±0.1091,分别高于对照组的0.1798±0.0476和0.0624±0.0342;咽管的NOS灰度值,实验组为0.3502±0.1310,高于对照组的0.2139±0.0603,差异有统计学意义(t=3.41,P<0.01)。肌纤维和口囊的SDH灰度值,实验组分别为0.1835±0.0771和0.4649±0.1039,与对照组间的差异无统计学意义(t=0.51,P>0.05)。结论密达利能抑制钉螺神经传导和能量代谢酶活性。
Objective To explore the killing mechanism of META-Li against Oncomelania hupensis by observing the change of enzyme activity in snail tissue. Methods Sixty snails were divided into 2 groups. Snails in experiment group were immersed in META-Li （100 mg/L） for 2d and soft tissue was separated for frozen sections, Histochemical staining for the enzymes CCO,LDH, SDH, AChE and NOS was done by routine method and the average grey density was measured under microscopy. Tissue sections of 10 snails were used to detect grey density for each enzyme. Snails without META-Li treatment served as control. Results The enzyme activity of CCO and AChE in the experiment group was significantly lower than that in the control （t=12.26, P〈0.01）, that of LDH and NOS in the experiment group was significantly higher than that in the control （t=3.41, P〈0.05）. There was no significant difference on the enzyme activity of SDH between the two groups （t=0.51, P〉0.05）. Conclusion The snail-killing effect of META-Li may be relevant to the enzyme activity in energy metabolism and the blocking of the nerve transmission.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2007年第3期198-201,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
密达利
钉螺
酶
组织化学
META-Li
Oncomelania hupensis
Enzyme
Histochemistry
