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依达拉奉对大鼠局灶性脑缺血再灌注损伤后JAK2/STAT3信号通路的影响 被引量：6

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摘要 目的探讨依达拉奉对大鼠局灶性脑缺血再灌注损伤后神经功能损伤、细胞凋亡及P-JAK2,P-STAT3蛋白表达的影响。方法雄性SD大鼠40只,随机分为假手术组、脑缺血再灌注组、生理盐水治疗组、依达拉奉治疗组,采用大脑中动脉线栓法制作大鼠局灶性脑缺血再灌注损伤模型,治疗组于脑缺血开始时及再灌注后12h分别腹腔注射依达拉奉3mg/kg或等量生理盐水,于24h后进行大鼠神经行为学评分;应用免疫组织化学及Western blotting检测P-JAK2,P-STAT3蛋白表达水平的变化;利用原位缺口末端标记法(TUNEL法)研究神经细胞凋亡的变化。结果与假手术组及生理盐水治疗组相比,依达拉奉治疗组大鼠神经行为学评分明显减少(P<0.01);P-JAK2,P-STAT3免疫阳性细胞及蛋白表达明显减少(P<0.01);凋亡细胞也减少(P<0.01)。结论依达拉奉尚能通过抑制与炎性细胞因子及氧化应激有密切关系的JAK2/STAT3信号转导通路显著减轻脑缺血再灌注损伤后神经损伤。
作者 谢惠芳 徐如祥 魏继鹏 姜晓丹 刘振华
机构地区 南方医科大学珠江医院神经内科 南方医科大学珠江医院神经外科
出处 《广东医学》 CAS CSCD 北大核心 2007年第7期1043-1046,共4页 Guangdong Medical Journal
关键词 脑缺血再灌注 依达拉奉 JAK2 STAT3 凋亡
分类号 R743.31 [医药卫生—神经病学与精神病学]
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参考文献10

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同被引文献90

  • 1何飞,杨峥嵘,谭颖徽.Notch配体Delta-1对人牙髓干细胞体外增殖的影响[J].牙体牙髓牙周病学杂志,2005,15(8):435-438. 被引量：6
  • 2谢惠芳,徐如祥,魏继鹏,姜晓丹,刘振华.大鼠脑缺血再灌注损伤后磷酸化JAK2、STAT3蛋白表达及细胞凋亡[J].南方医科大学学报,2007,27(2):208-211. 被引量：29
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引证文献6

二级引证文献30

广东医学
2007年 第7期

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