摘要
目的探讨微卫星不稳定性(MSI)在乳腺癌发生中的作用及其意义。方法收集40例乳腺癌新鲜标本,分离基因组DNA,通过PCR法扩增12个微卫星位点;银染方法显色,采用JEDA801E专业数码凝胶成像分析软件进行片段分析,观察12个位点重复序列长度的变化,确定乳腺癌MSI状态。结果乳腺癌MSI发生率为35%,良性增生均表现微卫星稳定;乳腺癌MSI发生率与分化程度有关,低分化乳腺癌MSI发生率明显高于高中分化组;乳腺癌中微卫星异常主要分布于D3S1766、D2S2739和TP533个位点。结论MSI是乳腺癌多步骤发展过程的早期事件;D3S1766、D2S2739和TP533个位点可能是检测乳腺癌MSI较敏感的位点。
Objective To investigate the incidence and clinicopathologic significance of MSI in breast caner. Methods 40 paired sporadic invasive breast cancer were collected. Genomic DNA was extracted from live sample. Twelve microsatellites on chromosomes 2p, 3p, 5q, 6q, 16q, 17q were amplified for MSI, respectively, by polymerase chain reaction （PCR） with designed primers and detecting after polyacrylamide gel electrophoresis. Results MSI in 15 out of 40 （35%） of the carcinomas were observed. There was no MSI in benign hyperplasia. MSI was mainly located at D3S1766, D2S2739 and TP53 in the breast cancer. The incidence rate of MSI in breast cancer is associated with the degree of carcinoma differentiation. Conclusions Microsatellite instability might play a role in the early stage during multistep breast carcinogenesis. MS1 indicated poor histologic differentiation in breast carcinoma. D3S1766, D2S2739 and TP53 might be the sensitive sites to detect MSI in breast carcinoma transformation.
出处
《医学研究杂志》
2007年第7期32-35,共4页
Journal of Medical Research
基金
浙江省教育厅基金资助项目(20020093)
关键词
乳腺癌
微卫星不稳定性
基因
Breast cancer
Microsatellite instability （MSI）
Gene
