摘要
通过RT-PCR克隆和测定了猪的谷氨酸脱羧酶-2(Glutamic acid decarboxylase 2,GAD2)基因的核苷酸序列并构建了该基因编码蛋白的三维结构分子模型。结果显示猪的谷氨酸脱羧酶-2基因的核苷酸长度由1758bp个碱基组成,编码产物为由585个氨基酸残基组成的多肽,编码产物中含有5-磷酸吡哆醛互作的结构域。构建包括共19物种的系统进化树,所得数据有利于我们了解谷氨酸脱羧酶趋同和趋异的进化途径。
The nucleic sequence of Glutamic acid decarboxylase 2 （GAD2） was cloned, sequenced using RT-PCR method and DNA sequencing techniques. Then the molecular model of three-dimensional structure of the predicted mature protein was constructed. The results revealed that the GAD2 gene was consistently 1758bp in length, encoding for a predicted polypeptide of 585 amino acid residues, including a pyridoxal-5＇-phosphate（PLP）. Multiple alignments were used to establish the primary structure homology for 19 glutamate decarboxylases of different origin, to design a phylogenetic tree, These data allowed us to elucidate the paths of divergent and convergent evolution of the enzyme.
出处
《中国农学通报》
CSCD
2007年第7期33-37,共5页
Chinese Agricultural Science Bulletin
基金
黑龙江省科技攻关项目"抑肌素基因剔除转基因猪的研究"(GB01B104)资助
