摘要
为了解克拉玛依地区婴儿利什曼原虫（Leishmaniainfantum）在猴体内的寄生特性，共用4只恒河猴（Macacamulatta）进行了研究，其中3只猴分别经颊部皮下接种从当地皮肤利什曼病患者的皮损组织以及从硕大白蛉吴氏亚种（Phlebotomusmajorwui）胃内分离出来的婴儿利什曼原虫，另一只猴作为对照。接种后观察局部皮肤的变化及对皮损组织作涂片检查或培养（NNN氏基），并于接种后1年～1年2个月解剖，作皮肤、肝、脾、淋巴结、骨及肾的组织学观察和涂片检查，肝、脾组织置NNN氏基内培养。结果发现，病人及蛉胃内的原虫对猴仅引起皮肤损害，而在皮损愈合后，原虫并未消失而是主要在接种部位的血管壁肌层内寄生繁殖。各内脏组织均未查见利什曼原虫。表明新疆克拉玛依地区的婴儿利什曼原虫对灵长类动物具有亲皮肤的特性。此一发现，对当地皮肤利什曼原虫复发的研究以及动物宿主的调查，均具有一定的意义。
In Karamay area of Xinjiang,Ieishmaniasis caused by L. infantum showed a distinct cutaneous lesion, but no clinical evidence of visceral damages was found in all cases. The aim of this paper is attempted to confirm the clinicopathologic form of leishmaniasis in Karamay area.Four rhesus monkeys were used,two of them were inoculated via cheeks with human origin of L. infantum 9～24 millions, the other one was infected via cheeks with sandfly origin of L. infantum 1O～2O millions,the remainder one was served as normal control. Twelve and fourteen months post inoculation, all animals were killed for pathological,tissue smear and culture examinations to detect the L. infantum.The results were as follows.1 Pathological observations 1.1 Gross observations: Half to 6 months after inoculation, the skin of 3 monkeys showed hyperaemia,swelling and even ulcer formation. After infection of 12～14 months,the cutaneous lesions mentioned above were found to be markedly lighten or almost disappearance. The visceras such as liver, spleen,mesenteric nodules, kidneys and bone showed normal in all infected monkeys.12 Microscopical observations: Six months after infection,the skin of rhesus monkey inoculated with sandfly origin of L. infantum,showed cutaneous tissue necrosis and ulcer formation,a great deal of leishmanias appeared in the inflammatory focus but only a few leishmanias were seen in the muscular layer of vessels in The project was supported by National Natural Science Foundation of China;WHO Collaboration Centre for Malaria, Schistosomias is and Filariasis.dermis. After inoculation of l2～14 months, the ulcerations of 3 infected monkeys were replaced by a dense connective tissue, the number of leishmanias in focus demonstrated obvious decrease in dermis. While in muscular layer of vessels which localized in dermis, a lot of leishmanias were investigated, the inflammatory reaction surrounded by vessels showed to be very light. No leishmanias or any histopathological changes were observed in visceras of 3 infected monkeys.2 Tissue smear and culture examinations The results of tissue smear and culture of liver and spleen to detect L- infantum in three infected monkeys were all negative.According to the above results, it suggested that, the cutaneous leishmaniasis caused by L. infantum in Karamay area was a simple form of cutaneous leishmaniasis. Besides above consideration,the authors also thought that the leishmanias parasitized in vessels might be the main cause of relapse in some cases of cutaneous leishmaniasis. The biological character of L. infantum which has a liking for vessels parasitism in rhesus monkey needs to be further research for explanation.
出处
《地方病通报》
1997年第1期8-9,共2页
Endemic Diseases Bulletin
基金
国家自然科学基金!39270628
关键词
婴儿
利什曼原虫
皮肤利什曼病
组织病理学
Leishmania infantum
Rhesus monkey
Cutaneous leishmaniasis
Histopathology
Parasitic character
