摘要
目的比较组织块法和细胞悬液法制作MDA-MB-231细胞乳腺癌原位移植型裸鼠模型的生物学特性与形态学特征。方法20只BALB/c小鼠随机分为两组,每组10只。将组织瘤块和细胞悬液分别接种于两组裸鼠右侧第二对乳腺的脂肪垫内,制作小鼠原位乳腺癌模型。比较两组的成瘤时间、成瘤率、肿瘤生长速度、肿瘤形态、肿瘤中心坏死、肿瘤表面血管分布情况和肿瘤表面溃疡出现时间,并观察肿瘤的组织学表现。结果两组的成瘤率无显著性差异。组织块法的成瘤时间早,瘤块形态欠规则,肿瘤血管少,易形成肿瘤中心坏死和表面溃疡;细胞悬液法的成瘤时间晚,瘤块形态规则,肿瘤血管丰富,中心坏死少,表面溃疡出现晚。病理学检查两组无显著差异。结论组织块法和细胞悬液法均可成功制作小鼠原位乳腺癌模型,其生物学特性较一致,形态学各有特点,可根据实验要求选择不同的制作方法建立动物模型。
Objective To analyze the biological characteristics and morphologic features between the mouse models using MDA-MB-231 cells established by injecting tumor cell suspension or implanting tumor tissues. Methods Twenty BALB/c mice were randomly divided into two groups, with 10 in each group. The orthotopic models of breast cancer were established by injection of tumor cell suspension or implantation of tumor tissues in the fat pad of right second breast in the nude mice of the two groups, respectively. The time of tumor formation, success rate, tumor growth rate, shape, central tumor necrosis, blood vessel distribution and tumor ulceration were compared between the two groups of models. The histological features of the tumors were also observed. Results There were no significant difference in the success rate between the two methods. In the group of tumor tissues implantation, the tumors formed in an earlier time with various shape configurations, and they were hypovascular and vulnerable to central necrosis with the resultant superficial ulcerations. However, in the group with injection of tumor cell suspension, the tumors formed later with a regular shape, and the superficial ulceration was rare and not vulnerable to central tumoral necrosis since the tumors were abundant in blood vessels resulting in adequate nourishment. There were no significant difference in the pathological findings between the two groups. Conclusion The nude mouse models of breast cancer can be successfully established by both tumor tissues implantation and tumor cell suspension injection, with identical biological characteristics and fairly different morphological features. The investigators are well advised to choose the more appropriate one according to the purpose of the experiments,
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2007年第7期818-821,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(30370428)
上海市基础研究重点项目(04JC14047)~~
