摘要
目的：比较不同采收时期栀子中栀子苷的含量，制定科学的采收时间。方法：采用RP—HPLC法，ODS柱，乙腈-水（15：85）为流动相，检测波长238nm。测定不同采收时期品种共5批栀子的栀子苷含量。结果：浓度在16．4—82．0μg·ml^-1范围内，栀子苷的进样量与峰面积呈良好的线形关系（r=0．9995，n=5），平均回收率为102．3％，RSD％=2．3％（n=5）结论：该法简便、准确，可为栀子的采收时期提供科学依据。
Objective： To compare the content of jasminoidin in gardenia at different culling period for setting up scientific culling period. Method： RPHPLC method was used, the chromatography consisted of ODS column with acetonitrile -water（ 15 ： 85 ） as mobile phase at the detection wavelength 238 nm. The jasminoidin in five batches of gardenias were determined at different culling stage. Result： When the concentration was at the range of 16.4 -82.0μg · ml^-1 , the sample size and peak area of jasminoidin showed a linear relatioship（r =0. 9995 ,n =5）. The average recovery was 102.3% with RSD% = 2.3% （n =5）, Conclusion： The method is simple, accurate, can supply scientific basis for culling period of gardenia.
出处
《中医药导报》
2007年第7期102-102,127,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
