摘要
目的对鼠疫F1抗原(抗体)ELISA检测试剂盒进行现场应用评价。方法结合鼠疫常规监测工作,对现场采集的标本同时进行常规鼠疫检验和ELISA检测,然后进行统计分析。结果鼠疫菌分离培养和ELISA检测人和动物脏器标本1798份,ELISA与鼠疫菌分离培养比较检出率较高(7.01%),符合率可达98.23%,鼠疫抗原ELISA检测试剂盒敏感性好、时间短。ELISA抗体检测试剂盒与间接血凝试验检测人和动物血清1份,结果无明显差异,但符合率低,ELISA加胶体金标记层析检测鼠疫F1抗体阳性可提高符合率(91.56%)。结论鼠疫F1抗原(抗体)ELISA检测试剂盒可以用于鼠疫的早期快速诊断。
Objective To evaluate application of the F1 antigen （antibody） ELISA test Kit in plague surveillence. Methods The samples from plague surveillance were detected by the routine method, ELISA and gold immuno chromatographic assay（GICA）, and statistical analysis their results. Results 1798 tissue samples of human and animals were detected with bacterial culture, ELISA and RIHA. ELISA detecting rate（7.01% ） were significantly higher than that of bacterial culture method（ P〈 0.01 ）, and their coincidence were 98.23%. 4401 serum samples of human and animals were detected with IHA, ELISA and GICA. ELISA detecting rate were no discrepancy with IHA（ P 〉 0.05 ）, hut the coincidence were low（61.04% ）. When ELISA and GICA were used together, the coincidence can be improved （91.56 % ）. Conclusion The F1 antigen（antibody） ELISA test kit for the diagnosis of plague is suit for the early detection of plague particularly in plague surveillence.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2007年第4期298-300,共3页
Chinese Journal of Vector Biology and Control
基金
国家"十五"攻关资助项目(2004BA718B07-01)
关键词
鼠疫
间接血凝试验
反向间接血凝试验
酶联免疫吸附试验
胶体金免疫层析
Yersinia pestis
Indirect hemoagglutination assay
Reverse indirect hemoagglutination test
Enzyme-linked imJnune sorbent assay： Gold immuno chromatographic assay
