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米曲霉GX0011 β-果糖基转移酶的分离纯化 被引量：2

Separation and Purification of β-fructosyltransferase from Aspergillus oryzae GX0011
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摘要 通过热变性、硫酸铵分级沉淀、DEAE-Sephadex A-50(柱1)、Octyl-Sepharose CL-4B、Sephacryl S-300、DEAE-Sephadex A-50(柱2)层析等分离纯化步骤对本实验室自行筛选诱变的米曲霉GX0011来源的β-果糖基转移酶进行了分离纯化,并通过SDS-PAGE检测了该酶的纯度和亚基分子量。结果表明电泳一条带,亚基表观分子量为5.1×104,小于多数文献报道的结果。纯化倍数为121.7,比活和活力回收分别为1975U-mg-1和17%。酶活测定表明该酶只起到转果糖基的作用而无水解活性。 The β-fructosyltransferase was separated and purified from the Aspergiilus oryzae GX0011 which was mutated and screened in the lab by using a successive procedure including heat-denaturing, ammonium sulphate precipitation, column chromatography separation by DEAE-Sephadex A50 （column 1）, Octyl-Sepharose CL-4B, Sephacryl S-300 and DEAE- Sephadex A50 （column 2）, respectively. The purified enzyme was confirmed to be homogeneous by SDS-PAGE and its molecular weight was estimated to be 5.1×10^4, which is smaller than that reported in literature. The final purified fold, specific activity and the yield of the purified enzyme are 121.7, 1975 U·mg^-1 and 17%, respectively. The obtained β-fructosyltransferase exhibits the catalytic activity of fructosyl transfer only and does not show any hydrolytic activity.
作者 覃益民 苏加坤 唐江涛 王机 姚评佳 魏远安
机构地区 广西大学化学化工学院 广西大学生命科学与技术学院
出处 《高校化学工程学报》 EI CAS CSCD 北大核心 2007年第4期710-714,共5页 Journal of Chemical Engineering of Chinese Universities
基金 国家自然科学基金资助项目(29772096) 广西高校博士点建设资金资助
关键词 低聚果糖 米曲霉 β-果糖基转移酶 分离纯化 fructooligosaccharides Aspergillus oryzae β-fructosyltransferase separation and purification
分类号 Q814.1 [生物学—生物工程]
  • 相关文献

参考文献18

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共引文献58

同被引文献26

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高校化学工程学报
2007年 第4期

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