摘要
为了探讨重组伪狂犬病毒TK-/gG-/ORF2+作为疫苗候选株的潜在价值,对该病毒的增殖特性、遗传稳定性、安全性、免疫原性及保护力进行了研究.结果表明:重组病毒的增殖滴度与亲本株相当,遗传稳定性良好,以100倍的免疫剂量接种小鼠时对小鼠是安全的;重组病毒在免疫小鼠后可诱导小鼠产生针对PRV和PCV2的特异性免疫应答,并且对致死剂量(100LD50)PRV Ea株强毒的攻击具有100%的免疫保护效果.
To study the potential value of a recombinant pseudorabies virus TK^-/gG^-/ORF2^＋ as candi- date vaccine, the biological characteristics of this virus were examined, including growth properties, genetic stability, security, immunogenicity and protective capacity, The growth properties of the recombinant virus in cells were similar to that of the parent vector virus. In animal experiments, TK^-/gG^-/ ORF2^＋ was safe to mice with dose of 100LD50 and elicited specific immune response against PRV and PCV2 in mice. Immunizat/on with TK^-/gG^-/ORF2^＋ protected mice against a lethal challenge （100LD50）with a virulent PRV Ea strain.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2007年第3期97-100,共4页
Journal of South China Agricultural University
基金
国家"863"高技术研究发展计划(2001AA213051)
关键词
重组伪狂犬病毒
增殖特性
遗传稳定性
安全性
免疫原性
保护力
recombinant pseudorabies virus
growth properties
genetic stability
security
immunogenicity
protective capacity
