摘要
用人类５号、９号、１３号、１５号、１７号、２０号整条染色体探针分别对人、恒河猴和食蟹猴的中期细胞进行荧光原位杂交，结果表明：人的５号、１３号、１７号探针分别杂交到恒河猴的５号、１６号、１７号染色体上；９号探针杂交到恒河猴１４号染色体的长臂及部分短臂上；１５号探针杂交到恒河猴７号染色体短臂及部分长臂上；２０号探针杂交到恒河猴的１３号染色体长臂上。食蟹猴的杂交结果与恒河猴完全一致。结合Ｇ带带型分析，对人与猕猴的染色体同源性及其进化进行了讨论。
Fluorescent in situ hybridization (FISH) was used on the metaphase chromosome of Macaca mulatta and Macaca fasicularis with human chromosome specific DNA libraries for chromosome 5、9、13、15 、17 and 20. In Macaca mulatta,the result showed that chromosome 5、16 and 17 was entirely painted by human chromosome 5 、13 and 17 specific libraries respectively. The long arm and the partial short arm of chromosome 14 and the short arm and the partial long arm of chromosome 7 were painted by human chromosome 9 and 15 specific libraries respectively. And the long arm of chromosome 13 was painted by human chromosome 20 library. The result was the same in Macaca fasicularis. Combinded with the comparative analysis of G-banding, the evolutional relationship of these chromosomes between human and macaques was discussed.
出处
《遗传》
CAS
CSCD
北大核心
1997年第3期9-12,共4页
Hereditas（Beijing)
基金
国家自然科学基金
关键词
荧光原位杂交
染色体
恒河猴
食蟹猴
FISH, Chromosome homology, Macaca mulatta, Macaca fasicularis
