摘要
以含6-BA、2,4-D培养基诱导蝴蝶兰外植体。6-BA可从嫩茎切块诱导出胚性愈伤组织，细胞富含淀粉粒，而2，4-D或2，4-D加6-BA诱导得到的愈伤组织不能分化。6-BA易于从嫩叶切片诱导胚性愈伤组织，进行体细胞胚发生过程的显微观察。在6-BA诱导嫩叶切片外植体期间，DNA含量前期平缓上升，第25d后急剧上升。RNA含量开始诱导就上升，第11-18d达到高峰。水溶性蛋白含量上升启动稍晚。在第25d达到高峰。
Two ex-plants from test tube seedling of Phalaenopsis sp. were cultured on the media containing 6-BA and 2, 4 -D. From the stem ex-plant, embryogenic callus （EC） with large amounts of starch granules in cell could be induced with the presence of 6-BA. On the other hand, the callus induced with 2, 4 - D or with both 2, 4 - D and 6- BA were unable to differentiate. EC could also be induced efficiently from the leaf ex-plant cultured on the medium containing 6 - BA. Histological observation and metabolic analysis conducted during somatic embryogenesis showed that, in leaf ex-plant, the DNA content increased slowly initially and sharply on the 25th day of induction. The RNA content increased when induction began, and reached a peak on the 11- 18th day. The soluble protein content started to change later than RNA and peaked on the 25th day.
出处
《福建农业学报》
CAS
2007年第2期162-166,共5页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(C0410040)
关键词
蝴蝶兰
细胞分裂素
体细胞胚
大分子代谢
Phalaenopsis sp
cytokinin
somatic embryogenesis
macromolecules metabolic kinetics
