摘要
本文报道用无毒性的活体荧光染料—钙黄绿素 AM标记的肿瘤细胞与人单核细胞共培养4小时,用Cyto Fluo 2300测定细胞粘附能力的荧光检测技术.结果表明IFN-γ和LPS激活的人单核细胞能迅速地与悬浮生长的人肿瘤细胞、B淋巴母细胞及淋巴细胞粘附.激活的人单核细胞表达更多的CD11a、CD11b、CD11c和CD18,对肿瘤细胞有更多的粘附性.抗体封闭及蛋白激酶抑制物处理细胞,粘附能力均受到明显的抑制.IFN-γ和LPS处理肿瘤细胞也能明显增加两种细胞间的粘附能力.上述结果提示人单核细胞粘附能力的增加与整合素及肿瘤细胞所表达的相关配体增加有关.
A new fluorescent method for testing the binding capacities of different cells was established. The target cells were labeled with non - toxic vital fluorescence dye,Calcein AM, then cocultured with human monocytes activated by rhIFN - 7 and IPS for 4hrs. The binding capacity was assayed with Cyto Fluor 2300 before and after removing unbinding target cells. The results showed that activated human monocytes could bind actively to the target cells especially tumorigenic cells. The expression of cellular adhesion moleculars (CAMs),especially CD 11/CD 18 on the human monocytes was dramatically increased after activated by LPS and rhIFN - γ. Blocking of CAMs with monoclonal antibodies would inhibit the binding capacities between the activated monocytes and tumorigenic target cells. The inhibitor of protein kinases, H - 7, also showed some inhibition to the binding capacities of these two kinds of cells. The binding capacities were also increased when target cells were pretreated with rhIFN - γ and LPS. These results indicated that integrins and their ligands are of very important functions in the immunological binding and recognition.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1997年第1期5-9,共5页
Chinese Journal of Cancer Biotherapy
关键词
粘附分子
整合素
单核细胞
肿瘤细胞
adhesion molecules
integrins
monocytes
tumor cells
Calcein AM
