摘要
以小麦叶肉细胞原生质体-激发子互作为研究体系,通过免疫荧光标记和Ca^(2+)荧光染料的装载并结合药理学试验,借助激光共聚焦扫描显微镜观察,探讨小麦抵抗叶锈菌侵染过程中微管骨架和Ca^(2+)之间的内在联系。试验结果表明,激发子处理可引起抗性品种原生质体[Ca^(2+)]_(cyt)的升高并诱发微管骨架的解聚,预解聚微管骨架,再用激发子处理,可使抗性品种原生质体[Ca^(2+)]_(cyt)的升高幅度增加。
By using immunofluorescence labeling technique and incubating the Fluo-3 AM of Ca^2＋ fluorescein, wheat（Triticum aestivum） cultivars Lovrin 10 and 5389 were selected as mesophyll protoplast material interaction with elicitor in this paper for studying the relationship between the pattern of microtubule and [Ca^2＋]cyt. Confocal Laser Scanning Microscopy（CLSM） was used and pharmacologic examination was set. The results indicated： when treated with elicitor, the cytosolic calcium in the protoplasts from the Lovrin 10 was increasing and the pattern of microtubule showed depolymerization. In the protoplasts which MTs were depolymerized by oryzalin prior to elicitor treatment, the increase of cytosolic calcium was even higher than that in the one which was not depolymerized.
出处
《分子细胞生物学报》
CSCD
北大核心
2007年第4期205-213,共9页
Journal of Molecular Cell Biology
基金
国家自然科学基金(30671244)
河北省自然科学基金重点资助项目(c2005000220
303180)
植物生理学与生物化学国家重点实验室开放课题资助项目(PPB04006)
教育部科学技术研究重点项目(03014)。
