摘要
目的探讨血小板生长因子(PDGF)在子痫前期(Preeclampsia,PRE)发病中的作用,进一步阐明PRE发病机制。方法研究对象为13例正常晚孕妇女,20例重度PRE晚孕患者。运用酶联免疫吸附法测定两组研究对象血清PDGF-BB含量;原位杂交技术检测两组胎盘蜕膜血管PDGF-B mRNA的表达。PDGF-BB血清含量及PDGF-B mRNA蜕膜血管表达强度灰度值间行直线相关分析。结果PDGF-BB含量在正常晚孕组为(39.61±18.20)pg/ml,在重度PRE晚孕组为(83.54±34.52)pg/ml,两组比较差异有显著性(P<0.001);重度PRE组胎盘蜕膜血管PDGF-B mRNA大量表达,强度明显高于对照组(P<0.001)。PDGF-BB血清含量及蜕膜血管表达强度灰度值呈明显负相关(r=-0.603,P<0.001)。结论PRE患者血清PDGF-BB明显升高,蜕膜血管PDGF-B mRNA表达增强,说明PDGF可能参与了PRE胎盘血管病变,参与PRE病情发生发展。血清PDGF-BB水平升高可能与局部病变的蜕膜血管有关。
Objective To study the role of platelet-derived growth factor （PDGF） in the pathogenesis of preeclampsia （PRE）. Methods Thirteen normal and 20 PRE late-pregnancy women were enrolled in this study. The serum PDGF-BB levels were measured with enzyme-linked immunosorbent assay, and the expression of PDGF-B mRNA in the decidual blood vessel was determined using in situ hybridization. Results PDGF-BB levels in PRE group was significantly higher than that in normal pregnant women （83.54±34.52 vs 39.61±18.20, P〈0.001）, and the expression ofPDGF-B mRNA in decidual blood vessel was also significantly higher in PRE group （P〈0.001）, showing a positive correlation between serum PDGF and PDGF-B mRNA expression （r=-0.603, P〈0.001）. Conclusion PDGF is associated with the pathology of decidual blood vessel. Elevated serum PDGF levels and PDGF-B mRNA expression in the decidual blood vessel may play an important role in the pathogenesis of PRE.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第8期1274-1276,共3页
Journal of Southern Medical University
关键词
子痫前期
血小板生长因子
胎盘
蜕膜
血管
preeclampsia
platelet-derived growth factor
placenta
decidua
blood vessels
