摘要
目的检测β-catenin在白血病患者中的表达,仞步探讨其在白血病中的作用。方法采用半定量 RT-PCR 方法检测β-catenin 在白血病患者中的表达,同时对部分标本应用免疫细胞化学检测其蛋白表达水平,结合临床资料分析其表达的意义。结果骨髓单个核细胞(MNC)中β-cateninmRNA 在急性髓系白血病(AML)和急性淋巴细胞白血病(ALL)患者中的表达(中位值分别为0.7593、0.6415)均明显高于正常人(0.3597)(P 值分别为0.001、0.016),也明显高于慢性粒细胞白血病(CML)慢性期患者(0.2571)(P 值分别为0.001、0.008),但存 AML 和 ALL 之间差异无统计学意义(P>0.05)。β-catenin 在 CML 慢性期患者的表达与正常人相比差异无统计学意义,但是在急变和加速期患者(0.9152)中明显增高,与急性白血病水平相当。按照 FAB 分型,β-catenin 在 AML M_5亚型中高表达,而在 M_3中表达明显低于其他亚型。免疫细胞化学结果显示,正常 MNC β-catenin 主要分布于细胞膜和细胞质,而在白血病细胞中,除 M_3外几乎均可以在细胞核中检测到其程度不一的表达。患者年龄、白细胞计数、高危核型以及治疗反应与β-catenin 均无显著相关,但在 AML 组β-catenin 与 CD34抗原表达有明显相关性。结论经典的 Wnt/β-catenin 信号传导途径有可能在急性白血病及 CML 急变和加速期中被激活。
Objective To investigate the expression of β-catenin in patients with leukemia and explore its significance in leukemias. Methods RT-PCR was used to detect the expression of β-catenin in bone marrow mononuclear cells （BMMNCs） from patients with leukemia. Immunocytochemistry was in some of patients to detect the distribution of β-catenin at the same time. The clinical significance of β-catenin was analyzed in combination with patients＇ clinical information. Results Expression of β-catenin was statistically higher in acute myeloid leukemia （AML） and acute lymphocytic leukemia （ALL） samples than in normal donors （ P = 0.001 and 0.016 respectively） and chronic phase chronic myeloid leukemia （CML） patients （ P = 0. 001 and P = 0. 008 respectively）, while there was no statistic difference between AML and ALL patients （P =0.58）. In addition, β-catenin expression in chronic phase CML patients was like that in normal donors （P = 0.49） , but increased significantly in blast crisis and accelerated phase. Immunocytochemical analysis revealed that BMMNCs from normal donors expressed β-catenin on the plasma membrane and cytoplasma, while those from acute leukemia expressed β-catenin to varying degrees in the nucleus as well. The expression of β-catenin gene statistically showed the highest level in M3 （ n = 15 ） and the lowest level in M3 （ n = 18 ）. No clinical features, such as, age, initial WBC count, therapy response rate, blast cell numbers or cytogenetic risk was found to be correlated with the expression of β-catenin excepting for CD34^＋ positive rate （ P = 0.004） in AML. Conclusion As a key mediator of Wnt signal transduction way, overexpression of β-catenin in leukemia cells indicates that it might be aberrantly activated in acute leukemia, accelerated or blastic phase of CML.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2007年第8期541-544,共4页
Chinese Journal of Hematology
基金
天津市自然科学基金(06YFJMSC08500)
人事部留学回国人员科技活动择优基金
