摘要
目的研究托吡酯(TPM)对大鼠脑缺血再灌注损伤的神经保护作用及机制。方法将健康成年Wistar大鼠40只随机分为正常对照组、假手术组、缺血再灌注组、TPM大剂量(100ms/kg)干预组及TPM小剂量(50ms/kg)干预组。分别在缺血再灌注72h后行神经功能评分,断头取脑,用免疫组化法检测脑组织NSE蛋白表达。结果与缺血再灌注组比较,TPM干预组神经功能评分、脑组织NSE表达明显增高(P<0.01);TPM大剂量干预组与小剂量干预组比较,神经功能评分、脑内NSE蛋白表达明显增加(P<0.05)。结论TPM对脑缺血再灌注损伤有神经保护作用,其机制可能为TPM增加缺血侧脑组织中NSE蛋白水平表达,其神经保护作用与给药剂量正相关。
[ Objective ] To study the expression and significance of neuron-specific enolase （NSE） in the cerebral tissue after cerebral ischemic reperfusion damage and elucidate the protective mechanism of topiramate in cerebral ischemic brain damage. [ Method] 40 Wistar rats were randomly assigned to sham operated group, ischemia-reperfusion group, TPM treatd group with large-dose and TPM treated group with small-dose. All the rats were killed to collect the brain in 72 hours after.the neural function value analysis were done. neurological grade evaluation was applied to examine the behavioral recovery and immunohistochemical technique was used to detect the dynamic changes of NSE in cerebral tissue. [ Result ] There was an improvement in the score of neurologic grade in topiramate treatment group in the 72th hour after reperfusion compared with the controlls（P 〈 0. 01 ）. The expression of NSE in topiramate treatment group increased than the fame- operation group in 72th hour after reperfusion （ P 〈 0. 01 ）. In treatment group, the score of the neurologic grade of the large-dose group increased（ P 〈 0. 05 ）, the expression of NSE protein increased remarkablely in cerebral tissue（ P 〈 0. 05 ） compared with the small-dose group. [ Conclusion] Topiramate could protect cerebral tissue against focal cerebral ischemic reperfusion damage whose role might be reflected by increasing the expression of NSE, and this neuroprotective effects is related to different dose of the medicine.
出处
《山东医药》
CAS
北大核心
2007年第22期5-6,共2页
Shandong Medical Journal
基金
山东省卫生科学技术发展计划项目(2002BB1DCA2)
