摘要
【目的】测定纯种大白猪(LW)、长白猪(L)、梅山猪(M)及其LW×L、L×LW、LW×M和M×LW等杂种猪血液和肌肉组织DNA甲基化含量,比较亲本与杂种DNA甲基化含量的差异,为揭示杂种优势的分子机制提供依据。【方法】采用高效液相色谱法测定血液和肌肉组织中甲基化含量。【结果】163份肌肉组织DNA样平均甲基化含量为16.92%,182份血液样平均甲基化含量为6.49%,两者之间差异达到了极显著水平(P<0.01),血液中DNA甲基化含量均低于10%,L×LW杂交猪最高,达到了9.93%,而长白猪最低,仅为2.97%;大白猪和长白猪之间差异极显著(P<0.01),而大白猪和梅山猪之间差异不显著(P>0.05);LW×L与L×LW杂种猪,LW×M与M×LW杂种猪之间均存在显著性差异(P<0.05)。肌肉组织中DNA甲基化含量3个纯种亲本之间无显著性差异(P>0.05);LW×L与L×LW杂种猪、LW×M与M×LW杂种猪之间差异均不显著,但是两个不同杂交组合,即LW×L、L×LW与LW×M、M×LW杂种猪之间差异显著(P<0.05)。【结论】本研究建立了测定猪血液和肌肉组织中DNA甲基化含量的高效液相色谱测定方法;DNA甲基化含量在血液和肌肉组织中差异显著,且具有组织特异性。
[Objective] In order to provide evidences for molecular mechanisms of heterosis in pigs, significant differences of DNA methylation contents between parental lines and their hybrids were analyzed both in blood and muscle tissues of pigs in parental lines of Large White （LW）, Landrace （L） and Meishan （M） and their hybrids LW × L, L × LW, LW × M and M × LW. [Method] High performance liquild chromatography（HPLC） was used in this study. [Result] The average DNA methylation content is 16.92% in 163 DNA samples of muscle tissue and 6.49% in 182 samples of blood, where the difference is especially prominent （P〈0.01）. The DNA methylation content is lower than 10% in all blood samples, varying from the highest 9.93% in L× LW hybrids to the lowest 2.97% in L parental lines. In parental lines, there is a very significant difference between LW and L （P〈 0.01）, but no significant difference between LW and Meishan pigs （P〉0.05）; in hybrids, the differences are significant between LW × L and L × LW （P〈 0.05）, as well as LW × M and M × LW （P〈 0.05）. As for the DNA methylation content in muscle tissues, there is no significant difference among the three parental lines （P〉0.05）; in hybrids, though there are no significant differences between LW × L and L × LW, as well as in LW × M and M × LW, there is a significant difference between the two different hybrid systems LW×L, L×LW and LW×M, M×LW （P〈0.05）. [Conclusion] A method of HPLC is developed for detecting DNA methylation contents in blood and muscle tissues of pigs. There are significant differences in average methylation contents and tissue specificity between blood and muscle tissue.
出处
《中国农业科学》
CAS
CSCD
北大核心
2007年第8期1774-1779,共6页
Scientia Agricultura Sinica
基金
国家自然科学基金(30371028)
