摘要
目的:观察黄芩苷对纯化培养乳鼠心肌细胞缺氧/复氧损伤的保护作用及其作用机制。方法:实验于2006-11/2007-01在郑州大学医学院药理实验室完成。①实验分组:选用出生1～3d的SD大鼠20只,雌雄不拘,培养乳鼠心肌细胞建立缺氧/复氧损伤模型。实验分为5组:正常对照组以正常细胞培养,加入等数量的培养基,不加入任何药物,缺氧/复氧损伤模型组,缺氧/复氧损伤+0.1mg/L黄芩苷组,缺氧/复氧损伤+1mg/L黄芩苷组,缺氧/复氧损伤+10mg/L黄芩苷组。②实验评估:采用乳酸脱氢酶试剂盒测其释放量;采用黄嘌呤氧化酶法测定超氧化物歧化酶活力;硫代巴比妥酸显色法测定丙二醛含量;四唑盐比色法测定心肌细胞活力。结果:①丙二醛含量和乳酸脱氢酶活力:缺氧/复氧损伤组明显高于正常对照组(P<0.01),缺氧/复氧+10,1,0.1mg/L浓度黄芩苷组明显低于缺氧/复氧损伤组(P<0.01)。②心肌细胞超氧化物歧化酶活性:缺氧/复氧损伤组明显低于正常对照组(P<0.01),黄芩苷+10,1,0.1mg/L浓度黄芩苷组明显高于缺氧/复氧损伤组(P<0.01)。③心肌细胞活力:缺氧/复氧损伤组心肌细胞活力明显低于正常对照组,缺氧/复氧+10,1,0.1mg/L浓度黄芩苷组明显高于缺氧/复氧损伤组(P<0.01)。结论:黄芩苷具有对缺氧/复氧损伤所致心肌细胞的保护作用,可能与其抗氧化、抗自由基有关。
AIM： To investigate the protective effect of baicalin on hypoxia/reoxygenation injury in cultured neonatal rat cardiomyocytes and the action mechanism. METHODS： The experiment was performed in the Department of Pharmacology, Medical College of Zhengzhou University from November 2006 to January 2007. ①Totally 20 SD rats aged 1-3 days of either sex were selected. Hypoxia/reoxygenation injured model was made with purified cultured cardiomyocytes, and the rats were divided into 5 groups, normal control group （normal cells were cultivated with only medium of the same volume）, hypoxia/reoxygenation injured group, hypoxia/reoxygenation plus low concentration baicalin group （0,1 mg/L baicalin）, hypoxia/reoxygenation plus middle concentration baicalin group （1 mg/L baicalin）, hypoxia/reoxygenation plus high concentration baicalin group （10 mg/L baicalin）. ②The releasing amounts, activities of superoxide dismutase （SOD）, contents of malondialdehyde （MDA） and activities of cardiomyocytes were measured with lactic dehydrogenase （LDH）, xanthine oxidase, thiobarbitudc acide and tetrazolium methods, respectively. RESULTS： ①Contents of MDA and activities of LDH were significantly higher in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups （P 〈 0,01）. ②The activities of SOD were significantly lower in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups （P 〈 0.01）. ③The viabilities of cardiomyocytes were significantly lower in the hypoxia/reoxygenation injured group than the normal control group, hypoxia/reoxygenation plus low, middle, high concentration baicalin groups （P 〈 0.01）. CONCLUSION： Baicalin has a protective effect on cardiomyocytes with hypoxia/reoxygenation injury, which can be associated with its antioxidation and anti-free radical effects.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第34期6765-6768,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
