摘要
目的探讨高血糖及胰岛素干预对大鼠脑缺血再灌注损伤的影响。方法将健康成年雄性SD大鼠160只随机分为假手术组、正常血糖组、高血糖组及胰岛素干预组,每组40只。高血糖组及胰岛素干预组采用STZ法诱导高血糖模型,以线栓法制作大脑中动脉缺血-再灌注模型,再灌注时间分别为1h、6h、12h和24h。大鼠清醒后行神经功能缺损程度评分(NSS)、TTC染色法计算梗死体积、HE染色和原位末端标记法(TUNEL)定量观察梗死周边区域凋亡神经元数目。结果与正常血糖组大鼠比较,再灌注同时间点高血糖组大鼠NSS显著增高(均P<0.01)、梗死体积明显扩大(均P<0.01)以及梗死周边区凋亡神经元数目明显增多(P<0.01);与高血糖组比较,胰岛素干预可明显控制血糖、减轻神经功能损害(12hP<0.05,24hP<0.01)、缩小梗死体积(均P<0.01)、减少神经元凋亡数(P<0.05～0.01),再灌注24h甚至可达正常血糖组水平。结论血糖水平升高可加重缺血再灌注性脑损害,可能与其诱导神经元凋亡有关,而胰岛素可能通过阻止神经元凋亡实现神经保护作用。
Objective To study the effects of hyperglycemia plasma glucose and insulin intervention on the cerebral injury after ischemia-reperfusion in rats. Methods 160 male SD rats were randomly divided into four groups： sham operation group, normal plasma glucose group, hyperglycemia group and insulin intervention group （40 rats in each group ）. The rats of hyperglycemia and insulin intervention groups were given STZ to induce hyperglycemia model. All the rats except for those in the sham operation group were subjected to right middle cerebral artery occlusion and then reperfusion. The reperfusion was started at 1 h, 6 h, 12 h and 24 h after 2 h ischemia. The neurologlc functional scores, infarct volume and numbers of neuron apoptosis of the rats were analyzed with the neurological severity score （NSS）, TTC staining assay and TUNEL method, respectively after the 2ats wake up. Results Compared with normal plasma glucose group, worse neurological function score, larger infarct size and more number of apoptotic neurons were found in hyperglycemia group at the same reperfusion time （all P 〈 0.01 ）. Moreover, these parameters in insulin intervention group were better than those in hyperglycemia group, and even similar with those in normal glucose group after 24 h of reperfusion. Conclusions Hyperglycemia can aggravate the cerebral injury of ischemia-reperfusion, perhaps by its effect of inducing neuron apoptosis o While insulin may play neuroprotective effect by inhibiting neuron apoptosis.
出处
《临床神经病学杂志》
CAS
北大核心
2007年第4期284-287,共4页
Journal of Clinical Neurology
基金
江苏省卫生厅"135工程"重点人才课题基金资助
关键词
高血糖
胰岛素
缺血再灌注
凋亡
hyperglycemia
insulin
ischemia-reperfusion
apoptosis
