摘要
利用农杆菌介导法将GhMADS3基因转入矮牵牛。对14株转基因植株进行分析鉴定,GUS染色表明外源基因在转基因植株中表达,PCR检测显示GhMADS3整合到矮牵牛的基因组中,RT-PCR分析表明GhMADS3在转基因植株中表达。转基因植株花器官普遍较野生型小,花萼膨大、顶尖向内弯翘,花冠深裂且边缘伴有不规则褶皱,柱头裸露在外,花器官颜色较野生型变淡甚至完全白化。测定花瓣、花萼中花色素苷和花萼中叶绿素含量,结果表明转基因植株均较对照偏低。说明GhMADS3的导入使得矮牵牛花形、花色发生改变。
An AGAMOUS homologue （ GhMADS3 ） was introduced into petunia via Agrobacterium tumefaciens mediated method. Forteen independent kanamycin-resistant petunia plants were alive. GUS staining revealed that ectopic gene was expressed in transgenic plants. PCR detection showed that the GhMADS3 was integrated into their genomes. The expression of the GhMADS3 was detected by RT-PCR. Constitutive expression of GhMADS3 resulted in visible phenotypic alterations in floral organ. In transgenic petunia plants, GhMADS3 resulted in the sepals swelling, corolla smaller and corolla deep spliting. It was noteworthy that the color of transgenic floral organ became weaker or entirely pale. The measuration of anthocyanin and chlorophyll showed that the content in transgenic plants were greatly lower than wild-type. It also indicated that GhMADS3 in plants has some relationship with the formation of flower color.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第4期985-990,共6页
Acta Horticulturae Sinica
基金
西南大学博士启动基金项目(2004博04)
