摘要
目的探讨氯胺酮在盲肠结扎穿孔术(CLP)脓毒症中的作用及可能的作用机制.方法120只雄性SD大鼠随机分为正常对照组、对照组、氯胺酮组;每组各分为Ⅰ、Ⅱ、Ⅲ、Ⅳ4个亚组.对照组和氯胺酮组建立CLP脓毒症模型.术毕半小时开始给药,氯胺酮组肌肉注射氯胺酮50 mg/kg,q 12 h,正常对照组与对照组给予等容积生理盐水,直至大鼠死亡或5 d观察期结束.每组的Ⅰ亚组大鼠观察5 d的生存情况;Ⅱ、Ⅲ、Ⅳ亚组的大鼠于术前0 h、术后4 h,及以对照组的Ⅳ亚组各只大鼠的临终时刻为观察时相点,分别处死每组中一个亚组的大鼠测定血中TNF-α、IL-6及NO的水平.结果与正常对照组比,对照组血清TNF-α、IL-6在CLP术后4 h及临终时点均明显升高(P<0.01);且TNF-α在CLP术后4 h较临终时点高;IL-6在临终时点较CLP术后4 h高;而NO在CLP术后的临终时点才明显升高(P<0.01).与对照组比,氯胺酮组血清TNF-α和IL-6在CLP术后4 h及临终时点均明显降低(P<0.01);NO仅在CLP术后的临终时点明显降低(P<0.01).正常对照组5 d生存率100%,对照组5 d生存率0,氯胺酮组5 d生存率60%;与对照组相比,氯胺酮组的5 d生存率明显升高(P<0.01).结论氯胺酮能够抑制CLP脓毒症大鼠血清TNF-α、IL-6和NO的水平,从而提高大鼠的生存率.
Objective To investigate the effects of ketamine on sepsis induced by cecal ligation - perforation （CLP） and the possible underlying mechanism. Methods 120 male SD rats were randomly allocated into three groups： normal control group, control group, and ketamine group. Each group was divided into four subgroups （ Ⅰ,Ⅱ,Ⅲ,Ⅳ ）. A cecum ligation and puncture procedure was used to make sepsis model. After half an hour of operation, ketamine group was administered with ketamine 50 mg/kg every 12 h intramuscularly, whereas normal control group and control group were given equivalent volume normal saline until rats died or 5 - day observation ended. The survival period was observed in each subgroupsⅠ during 5 days. Arterial blood samples were taken from rats in subgroups Ⅱ,Ⅲ and IVfor measurements of tumor necrosis factor-α （TNF-α） , interleukin - 6 （ IL - 6 ） and nitric oxide （ NO ） at 0 hour before operation, 4 hours after operation and the dying point of each rat of control subgroup Ⅳ. Results In control group TNF -α and IL - 6 significantly elevated after the CLP procedure compared with those in normal control group （P 〈0.01）. Moreover TNF-α cot,cot, at 4h after the CLP procedure was higher than that at the dying point; IL -6 levels increased in parallel with the time elapsed. NO in control group only significantly elevated at the dying point compared with that in normal control group （P 〈0.01 ）. Decreases in the levels of TNF -α and IL -6 were found at anytime after ketamine intervention （P 〈0.01 ） ; NO in ketamine group significantly decreased at the dying point compared with that in control group （P 〈0.01）. The 5 -day survival rate in normal control group was 100%, in control group was 0, in ketamine group was 60%. A significantly higher survival rate was found in ketamine group than that in control group （ P 〈 0.01 ）. Conclusion Ketamine could restrain the TNF -α, IL - 6 and NO serum concentrations by the CLP procedure and raise the survival rate of rats with sepsis.
出处
《昆明医学院学报》
2007年第4期79-83,共5页
Journal of Kunming Medical College
