摘要
目的探讨在门静脉转流下梗阻性黄疸大鼠门静脉缺血后肝脏能量代谢变化的病理特征及其与动物耐受性的关系。方法在门静脉转流下阻断门静脉不同时间后,观察梗阻性黄疸大鼠存活率、肝细胞线粒体呼吸活性、肝组织ATP含量及动脉血酮比值。结果门静脉缺血30、60及90 min后7 d大鼠存活率分别为100%、100%及40%,缺血后肝脏能量代谢功能明显受损,在再灌注后24 h,门静脉缺血30 min及60 min 2组大鼠肝脏能量代谢功能已有明显恢复,而门静脉缺血90 min组肝脏能量代谢功能仍维持在显著低水平。结论在门静脉转流下梗阻性黄疸大鼠门静脉缺血60 min以内肝脏能量代谢损害可逆,而门静脉缺血90 min引起梗阻性黄疸大鼠肝脏能量代谢功能不可逆损害,大鼠难以耐受。
Objective To study the pathological changes of hepatic energy metabolism following portal vein ligation and the tolerant limit to ischemia inrats with obstructive jaundice and portal flow bypass. Methods Survival rate, hepatocytic mitochondrial respiratory function and ATP content, ketone body ratio in arterial blood were investigated in different duration of portal vein ischemia and reperfusion with portal blood bypass. Results The survival rate on 7th postoperative day was 100%, 100% and 40% when subjected to 60, 90 and 120 minutes of portal flow occlusion. The hepatic energy metabolic function decreased markedly after ischemia, and increased evidently at 24 - hour postreperfusion subjected to 60, 90 minutes of portal flow occlusion, but less recovery was observed in the rats with 120 minutes of portal flow occlusion. Conclusion The damage of hepatic energy metabolic function was reversible in rats with portal flow occlusion within 90 minutes, but irreversible for those with 120 of portal flow occlusion.
出处
《实用临床医药杂志》
CAS
2007年第4期35-38,共4页
Journal of Clinical Medicine in Practice
