摘要
用发根农杆菌１５８３４，ＬＢＡ９４０２株和根癌农杆菌Ｃ５８株感染丹参无菌苗，完成质粒转化后诱导出毛状根和冠瘿瘤。毛状根和冠瘿组织在无激素的培养基上于光照条件下分化出转化后的丹参再生植物，再生植物移植到土壤中能够成活。用发根农杆菌转化后的再生植物的具有节间缩短，植株矮化、地下部毛状须根发达等特征。用根癌农杆菌转化后的再生植物生长旺盛，地上部分较原植物高大，根系发达，根产量和丹参酮含量都高于原植物。
The hairy roots and crown galls of Salvia miltiorrhiza were obtained by infecting plant with A. rhizogenes (strain 15834, LBA 9402)and A. tumefaciens (strain C 58 ). The transformed plants were regenerated light and transplanted form cultural medium into soil successfully. The plants transformed by A.rhizogenes have characteristics of short stems and develop hairy roots, those and trans formed by A.tumefaciens grow vigorously featuring, longer stems and well developed roots. Both biomass production and tanshenone content are higher than the original plant.
出处
《中国中药杂志》
CAS
CSCD
北大核心
1997年第5期274-275,共2页
China Journal of Chinese Materia Medica
基金
国家自然科学基金
关键词
农杆菌转化
丹参
再生植物
transformation by Agrobacterium
plant regeneration
