摘要
目的:分析比较小分子干扰RNA和反义寡核苷酸技术的作用机制、作用靶点、设计方法和临床应用的异同。资料来源:应用计算机检索PubMed数据库1990-01/2007-02相关小分子干扰RNA和反义寡核苷酸方面的文献,检索词"siRNA;accessible site;antisense oligonucleotides;RNA Interference;mechanism;chemically modified siRNA;chemically modified oligonucleotides",限定文献语言种类为English。资料选择:对资料进行初审,选取有关小分子干扰RNA和反义寡核苷酸的文献,开始查找全文。纳入标准:关于RNA干扰和反义寡核苷酸作用机制的研究;探讨如何在mRNA上寻找小分子干扰RNA和反义寡核苷酸作用靶点的论文;解释反义寡核苷酸化学修饰和小分子干扰RNA分类的文章;应用小分子干扰RNA治疗肿瘤的探索性研究。排除标准:非原创性的文章。资料提炼:共检索到200多篇关于小分子干扰RNA和反义寡核苷酸的文献,最终纳入40篇符合标准的文献。资料综合:尽管小分子干扰RNA和反义寡核苷酸作用机制不尽相同,但两者在很多方面如选择mRNA的作用靶点、化学修饰和应用等方面,是相通的。研究反义寡核苷酸的经验可以使小分子干扰RNA的研究事半功倍。本文对小分子干扰RNA和反义寡核苷酸技术进行了比较,这将有助于小分子干扰RNA的研究与应用。结论:研究反义寡核苷酸技术的经验对小分子干扰RNA的研究有指导意义,小分子干扰RNA是非常有希望被用于肿瘤治疗的新型药物。
OBJECTIVE： To analyze the differences in the action mechanism, accessible sites, design and clinical application between small interfering RNAs （siRNAs） and antisense oligonucleotides. DATA SOURCES： Online search of PubMed database was undertaken to identify the articles about siRNAs and antisense oligonucleotides published in English between January 1990 and February 2007 with the key words of ＂siRNA, accessible site, antisense oligonucleotides, RNA Interference, mechanism, chemically modified siRNA; chemically modified oligonucleotides＂. STUDY SELECTION： The data were selected firstly to choose those about siRNAs and antisense oligonucleotides, and the full-texts were looked up. Inclusive criteria： Research about the action mechanism of RNA interference and antisense oligonucleotides; papers on the search of siRNAs on mRNA and the accessible site of antisense oligonucleotides; the chemical modification of antisense oligonucleotides and siRNAs; exploratory studies on the application of siRNAs in treating tumor. Exclusive criteria： unoriginal articles. DATA EXTRACTION： More than 200 related articles were collected and 40 ones in accordance with the inclusive standards were selected. DATA SYNTHESIS： Although the action mechanism of siRNAs is different from that of antisense oligonucleotides, they are greatly related in the selection of accessible sites in targeted mRNAs, chemical modification and application. The experiences in studying antisense oligonucleotides facilitate the researches on siRNAs. In this article, the siRNA and antisense oligonucleotides are compared to help the research and application of siRNAs. CONCLUSION： The experiences of researches on antisense oligonucleotides could guide the researches on siRNAs, which is a promising new type medicine for tumor.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第36期7262-7266,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金委员会资助项目(0500132)~~
