摘要
目的:探讨青少年特发性脊柱侧凸(AIS)患者椎旁肌钙调蛋白的表达及其对肌浆网钙离子通道的影响。方法:AIS患者共30例,Cobb角40°～100°,其中Cobb角≥50°者15例(A组),Cobb角<50°者15例(B组);对照组(C组)5例患者均为非脊柱侧凸病例。于手术中取A、B组患者顶椎区两侧椎旁肌、取C组患者非病变部位两侧椎旁肌作为标本进行分子生物学分析,采用RT-PCR方法检测所有标本钙调蛋白和肌浆网钙离子通道的mRNA表达量。结果:A、B组患者椎旁肌钙调蛋白mRNA的表达比C组高(P<0.05),并且A组患者凸侧椎旁肌钙调蛋白mRNA显著高于凹侧(P<0.05),而B组患者其凹凸侧钙调蛋白mRNA没有显著性差异(P>0.05);C组椎旁肌肌浆网钙离子通道mRNA的表达比A组患者高(P<0.05),A组患者凸侧椎旁肌肌浆网钙离子通道mRNA显著低于凹侧(P<0.05),而B组患者其凹凸侧肌浆网钙离子通道mRNA没有显著性差异。结论:钙调蛋白mRNA在AIS患者椎旁肌中的表达较无脊柱侧凸者高,而且其表达随着侧凸程度的加重而增高;AIS患者椎旁肌肌浆网钙离子通道表达有异常,钙调蛋白的过度表达可能导致了钙离子通道的损伤。
Objective：To study the changes of calmodulin and ryanodine receptor 1 mRNA expression in paravertebral muscles in adolescent idiopathic scoliosis （AIS）.Method：There were 2 groups：AIS group had 30 cases （2 males,28 females,11-19 years old,Cobb angle 40°-100°）,including 15 cases with Cobb angle≥50°（group A） and 15 cases with Cobb angle〈50°（group B）.Control group had 5 cases without scoliosis（group C）.The paravertebral muscles in apex vertebral region in group A/B and in non-diseased region in group C were harvested and analyzed.The mRNA expression of calmodulin and ryanodine receptor 1 was detected by RT-PCR method.Result：The calmodulin mRNA expression in paravertebral muscles in group A/B was more than that of the paravertebral muscles in group C（P〈0.05）,and the calmodulin mRNA expression in the convex side paravertebral muscles was significantly more than that of the concave side in group A （P〈0.05）,but the calmodulin mRNA expression showed no significant difference in both side in group B（P〉0.05）.The ryanodine receptor 1 mRNA expression in paravertebral muscles in group C was more than that of the paravertebral muscles in group A （P〈0.05）,and the ryanodine receptor 1 mRNA expression in the convex side paravertebral muscles was significantly lower than that of the concave side in group A （P〈0.05）,but the ryanodine receptor 1 mRNA expression showed no significant difference in both side in group B （P〉0.05）.Conclusion：The calmodulin mRNA expression in paravertebral muscles in AIS patients are more than that of the paravertebral muscles in non-scoliosis people,and the level of calmodulin is associated with the severity of the spinal curve.The ryanodine receptor 1 mRNA expression in paravertebral muscles in AIS patients is abnormal,which may due to the over expression of calmodulin.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2007年第9期684-688,共5页
Chinese Journal of Spine and Spinal Cord
