摘要
目的:研究川芎嗪注射液对博莱霉素A5致肺纤维化大鼠肺组织Ⅰ型胶原(Col-Ⅰ)、Ⅲ型胶原(Col-Ⅲ)及层粘连蛋白(LN)的干预作用。方法:70只Wistar大鼠随机分为空白组、模型组、川芎嗪大剂量组(简称"大剂量组")、川芎嗪中剂量组(简称"中剂量组")、川芎嗪小剂量组(简称"小剂量组")。除空白组外其他4组气管内注入博莱霉素A5造模,造模第2天大、中、小剂量组开始每日腹腔注射川芎嗪注射液至第28天,剂量分别为250 mg.kg.d-1、150mg.kg-1.d-1、40mg.kg-1.d-1。第28天时采用免疫组织化学方法检测肺组织Col-Ⅰ、Col-Ⅲ、LN的表达,并进行半定量分析,同时取肺组织行HE染色,观察病理形态学改变。结果:与空白组比较,模型组Col-Ⅰ、Col-Ⅲ及LN相对含量明显升高(P<0.01);川芎嗪各剂量组表达有不同程度的降低,以大剂量组最为明显。结论:川芎嗪注射液能抑制肺纤维化大鼠Col-Ⅰ、Col-Ⅲ、LN的表达,减少细胞外基质积聚,这可能是川芎嗪注射液发挥治疗作用的机制之一。
Objective： To study the role of ligustrazine injection on collectionⅠ（Col-Ⅰ）,collection Ⅲ（Col-Ⅲ） and laminin（LN）in pulmonary fibrosis rats induceded by bleomycin A5.Methods： Seventy Wistar rats were divided into 5 groups at random： normal group;model large-dose group;middle-dose;little-dose group.The other groups were injected bleomycin A5 in trachea except for normal group.From the second day,large-dose group,middle-dose group,little-dose group were given ligustrazine injection by intraperitoneal injection to 28th day,the dose was dividedly： 250,150,40 mg·kg^-1·d^-1.Col-Ⅰ,Col-Ⅲ and LN in lung tissue were detected in 28th day by immunohistochemistry,and the semiquantitative analyses of them were detecrted.HE staining was detected and the pathomorphology were observed.Results： The contents of Col-Ⅰ,Col-Ⅲand LN of lung tissue in model group rats were significantly upgraded compared with that of normal group（P〈0.01）;Large-dose group,middel-dose and little-dose group could degrade the content of Col-Ⅰ,Col-Ⅲ and LN in lung tissue.However, the effect of large-group was more effective.Conclusion： It is one of mechanisms to ligustrazine injection＇s therapeutic action to degrade the content of Col-Ⅰ,Col-Ⅲ and LN in lung tissue and extracellular matrix accumulation of pulmonary fibrosis rats.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2007年第9期613-615,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
河北省科学技术厅科技攻关项目(No.052761117)
关键词
川芎嗪
肺纤维化
病理形态学
细胞外基质
动物实验研究
Ligustrazine
Lung tissue
Pathomorphology extracellular matrix
Animal exprimental study
