摘要
将Ｃ５７ＢＬ／６小鼠脾淋巴细胞分成四组：①单纯培养液组；②单纯ＰＡ组；③ｒＩＬ－２组；④ｒＩＬ－２＋ＰＡ组。采用ＭＴＴ比色方法及间接免疫荧光法测定并比较各组淋巴细胞增殖反应，淋巴细胞对ｐ８１５肿瘤细胞的细胞毒作用以及淋巴细胞表面ＩＬ－２表达的情况，以此观察ＰＡ体外对小鼠脾ＬＡＫ细胞增殖反应、杀瘤活性及ＩＬ－２Ｒ表达的影响。结果表明，ＰＡ单独对静止淋巴细胞增殖反应无作用，也不能诱导出ＬＡＫ活性，但能显著增强ＩＬ－２诱导的淋巴细胞增殖反应，杀瘤活性及其表面ＩＬ－２Ｒ表达。当浓度为１０μｇ／ｍ１的ＰＡ与低剂量（２００ｕ／ｍ１）ＩＬ－２联合应用时，诱导出的ＬＡＫ活性强度相当于浓度为１０００ｕ／ｍ１ＩＬ－２水平。提示，ＰＡ与ＩＬ－２合用能共同诱导ＬＡＫ活性，降低ＩＬ－２用量，ＰＡ可望作为一种新的免疫调节剂用于肿瘤的ＬＡＫ／ＩＬ－２生物治疗。
In order to observe the effect of invitro PA on the proliferation, cytotoxicity and IL 2R expression, spleen cells of C57BL/6 mice were randomly divided into four groups. One served as control, the other three groups were incubated with PA, IL 2, and combination of PA and IL 2, respectively. We measured and compared the proliferation, cytotoxicity for p851 and IL 2 receptor expression of each group spleen cells by using MTT colorimetric assay and indirect immunofluorescence. The results demonstrate that PA alone is not capalbe of inducing LAK activity, but can significantly enhance the proliferation, IL 2R expression of LAK cells in the presence of IL 2. Combination of PA(10 μg/m1) and low dose IL 2 (200 u/ml) can induce the same high indue LAK cytofoxicity, decrease the dose of IL 2(1 000 u/ml) alone. These resuts snggest that combination of PA and IL 2 can indue LAK cytofoxicity, decrease the dose of IL 2, and may be used as a near immunomdouator in LAK/IL 2 tumor biotherapy.
出处
《实用癌症杂志》
1997年第2期102-104,共3页
The Practical Journal of Cancer
关键词
多抗甲素
白细胞介素2
杀伤细胞
LAK
Polyactin A(PA) Interleukin 2 Lymphodine cativated killer(LAK) In vitro
