摘要
目的探讨人参农家类型的遗传多样性和亲缘关系,为人参的栽培和选育种提供遗传学依据。方法采用ISSR分子标记分析5种人参农家类型7个居群120个样本的遗传多样性。结果人参农家类型有较丰富的遗传多样性,平均多态位点百分率为48.85%;不同农家类型的遗传多样性水平有差异,和其他农家类型相比,长脖和竹节芦的遗传变异较小,且两者之间的相似性系数高达97%;不同产地的同一人参农家类型间也存在很大的遗传差异。结论研究表明人参农家类型的遗传差异主要存在于各类型内部,而且可能更多地存在于同一类型的不同居群内部。为促进人参新品种的选育,有必要在现有的栽培群体中补充不同产地的同一类型的种质资源。
Objective To discuss the genetic diversity and relationship of Panax ginseng landraces and provide genetic references for ginseng cultivation, selection and breeding. Methods The genetic diversity of 120 individuals from seven populations, representing five P. ginseng landraces, was analyzed by intersimple sequence repeat （ISSR） markers. Results P. ginseng landraces held higher genetic diversity and the average percentage of polymorphic loci was 48.85 %. Genetic diversity levels were very different among various landraces. The landraces of Zhujielu and Changbo showed relatively low genetic variation as compared with the others and their similarity coefficient was up to 97%. A large genetic variation existed in the same landraces but cultivated in different habitats. Conclusion This study indicates that the large genetic variations mainly exists within each landrace, but even more variations might exist within different populations from a same race. For promoting the breeding and selecting of P. ginseng varieties, it is necessary to supply germplasm of the same race from different habitats to the currently cultivated populations.
出处
《中草药》
CAS
CSCD
北大核心
2007年第9期1392-1395,共4页
Chinese Traditional and Herbal Drugs
基金
上海市科委中药现代化专项(03DZ19547
04DZ19834)
关键词
人参
农家类型
遗传多样性
ISSR
Panax ginseng C. A. Meyer
landrace
genetic diversity
ISSR
