摘要
采用8%和10%两个浓度梯度的SDS-聚丙烯酰胺凝胶电泳,对黄花苜蓿、天蓝苜蓿、一年生苜蓿(Orion)、紫花苜蓿(敖汉)及1个紫花苜蓿杂交种共5种不同苜蓿的种子可溶性蛋白和盐溶蛋白图谱进行了研究。结果显示:5个种10%和8%凝胶电泳的种子可溶性蛋白总带数分别为97和91,10%凝胶电泳的种子盐溶蛋白共有69条谱带;5种苜蓿在种子可溶性蛋白和盐溶蛋白电泳图上都具有各自的特征谱带,谱带数目、位置、宽窄和颜色深浅都存在明显的差异;10%凝胶的可溶性蛋白指纹图谱谱带数目相对较多,种间遗传差异在A、B、C区均有分布,谱带的重复性好,是鉴别种的最好图谱。5种苜蓿种子可溶性蛋白和盐溶蛋白指纹图谱一致,表明杂交种和敖汉苜蓿的谱带相似性最大,与黄花苜蓿的相似性次之,而这3个种与天蓝苜蓿和一年生苜蓿(Orion)的相似性较小。这与种在形态学上表现出来的差异相一致,从而进一步肯定了采用种子贮藏蛋白电泳技术进行苜蓿分类研究和种子鉴别的可靠性。
Using 10% and 8%gel SDS-PAGE, the dissoluble proteins and seed salt soluble proteins in seeds of five alfalfa species, which include Medicago falcata, Medicago lupulina, Medicago sphaerocarpos cv. ‘Orion＇, Medicago sativa cv. ‘Aohan＇, and one hybrid Medicago sativa ×Medicago ruthenica were analyzed. The results showed that the total dissoluble proteins bands of 8% and 10% gel were 91 and 97, respectively, and the seed salt soluble proteins bands of 10% gel were 69; All of the five alfalfa species have their own characteristic bands, which are different in number, distribution, width, and concentration; Among three methods, the five species‘ seed dissoluble protein fingerprints of 10% gel＇ bands is relatively more, which genetic diversity scattered in A , B and C regions, and can be repeated in different experiments. SO it＇s the best fingerprint. The fingerprints of the seed dissoluble proteins and seed salt soluble proteins of five species are unanimous, which showed that： the hybrid has the nearest genetic distance with Medicago sativa cv. ‘Aohan＇, and Medicago falcata is nearer. But the three species have wide genetic distance with Medicago lupulina and Medicago sphaerocarpos cv. ‘Orion＇. The results of species identification using fingerprints was the same as the morphology, so it further affirmed the dependability using the seed storage protein electrophoresis to classify the Medicago and differentiate the seeds.
出处
《中国草地学报》
CSCD
2007年第5期1-7,共7页
Chinese Journal of Grassland
基金
农业结构调整重大技术研究专项(04-13-02B)
国家重点基础研究发展计划(2007CB106800)
农业部草地资源生态重点实验室资助项目(23269208)
关键词
苜蓿
种子贮藏蛋白
指纹图谱
Alfalfa
Seed storage proteins
Fingerprints
