摘要
应用流式细胞术检测大鼠腹腔感染经CF-11纤维素纯化的活弓形虫速殖子悬液后60 d内大鼠外周血CD3+、CD4+、CD8+T淋巴细胞的百分率,并应用酶联免疫吸附试验测定其外周血IFN-γ、TNF-α、IL-4等细胞因子的水平。结果显示:CD8+T细胞亚群水平在感染弓形虫后3 d、7 d、14 d和21 d时均显著低于正常水平(P<0.05),至感染后28 d恢复至正常;而CD4+T细胞亚群水平在整个感染过程中并无显著变化;CD4+/CD8+比值升高,以感染后14 d最为明显;大鼠外周血中IFN-γ和IL-4水平在感染后7 d开始升高(P<0.05),至感染后60 d时仍高于正常水平,TNF-α至感染后28 d时高于正常水平(P<0.05),至感染后60 d时仍高于正常水平。表明大鼠感染弓形虫对其免疫功能有一定程度的影响。
T lymphocytes play a key role in anti-infectious immunological response. In order to investigate the immunity of T lymphocytes of rat after being infected by Toxoplasma gondii, flow diagnosis of T cell subpopulations and ELISA of the levels of cytokines IFN-γ, TNF-α and IL-4 in the serum of rat infected with tachyzoites suspension of Toxoplasma gondii purified by cellulose CF-11 were performed. The amount of T cell subpopulation CD8 ^＋ in the peripheral blood of infected rat was lower than （P 〈0. 05 ） that in non-infected rat on day 3, day 7, day 14 and day 21 of infection, and was increased to normal on day 28. Another subpopulation CD4 ^＋ did not exhibit significant changes, however, the ratio of CD4^＋ to CD8 ^＋ rose to the highest on day 14 of infection. The elevation （P 〈0. 05 ） of the contents of cytokines IFN-γ, and IL-4 began on day 7 of infection and lasted to day 60. The content of IL-4 was found increased （ P 〈 0. 05 ） from day 28 to day 60 as well. Based on the results observed above, it was elucidated that the infection by Toxoplasma gondii could affect the immunological responses of rat.
出处
《江苏农业学报》
CSCD
北大核心
2007年第4期322-325,共4页
Jiangsu Journal of Agricultural Sciences
基金
江苏省人口计划生育委员会科研基金项目
