摘要
在云南红豆杉细胞悬浮培养中，适宜的培养基为B5，接种量为0．5～0．8g干重细胞／100ml培养基，2，4－D浓度为1．0mg／L；培养细胞的生长周期约30d；培养基中较高浓度的蔗糖（40g／L）可提高紫杉醇含量；添加的椰子汁（CM）、酪蛋白氨基酸（C）和水解乳蛋白（LH）3种有机添加剂均能提高培养细胞中紫杉醇的含量，但只有CM和CA能促进细胞的生长。于B5培养基中添加不同浓度的NH4NO3对培养细胞无明显影响。
B5 medium was suitable for cell suspension cultUre of Taxus yunnanensis (Fig. 1 ). The growth cycle of suspension cells was ahaut 30 days (Fig. 2 ).The ophmum size of inoculum and 2, 4D concentrahon for cell growth and taxol content was 0. 5～0. 8 g (cell DW)/100 ml medium(Fig. 3) and 1. 0 mg/L(Fig. 4) respechvely. Taxol content could be Promoted by increasing the sucrose concentrahon to 40 g/L (Fig.5), and also by supplemenhng with organic substances like coconut balk (CM), caseinhydrolysat(CA) and lactalbuthen hydrolysate (LH) (Fig. 7 ).Only CM and CA Promoted cell growth of culture cells. Effects of different concentrahons of KNOb on cell suspension culture of Taxus yunnanensis were not significant(Fig. 6).
基金
中国科学院"八五"重点科研项目
关键词
云南红豆杉
悬浮培养
细胞培养
Taxus yunnanensis
cell suspension culture
taxol
