摘要
目的研究犬脊髓损伤后致神经源性膀胱逼尿肌中毒蕈碱样胆碱受体亚型(M2)受体的变化,探讨M2受体在犬神经源性膀胱逼尿肌的意义。方法建立动物模型,设骶上型、骶下型神经源性膀胱组和对照组,RT-PCR法半定量检测膀胱体部逼尿肌M2受体mRNA。结果骶上型组膀胱容量(281.0±26.2)mL,逼尿肌压力(58.5±5.7)cmH2O,膀胱顺应性(4.8±1.1)mL/cmH2O;骶下型组容量(513.2±44.4)mL,逼尿肌压力(40.3±4.1)cmH2O,顺应性(12.2±2.1)mL/cmH2O,2组比较有统计学意义(P<0.05)。对照组逼尿肌压力(43.0±3.9)与骶下组比较无统计学意义。对照组膀胱逼尿肌有M2受体mRNA表达,骶上型组和骶下型组M2受体mR-NA较对照组增加(P<0.05),骶上型组明显高于骶下型组(P<0.05)。结论脊髓损伤后神经源性膀胱逼尿肌M2受体增多,骶上型M2受体增多更明显,M2受体可能直接参与骶上型神经源性膀胱逼尿肌无抑制性收缩。
Objective To study the alteration of the M2 muscarinic receptors in the detrusor of neurogenic bladder after spinal cord injury, and the pathophysiology of the M2 muscarinic receptors in the bladder＇s detrusor of the canine model. Methods The canine neurogenic bladder model were performed by operative destracion of L4-6 nerve. The mRNA of the M2 muscarinic receptors was detected by RTPCR. Results The volume of the bladder in the suprasacral group was （281±26.20）mL; the pressure of the detrusor was （58.5±5.7）cmH2O. the compliance of the bladder was （4.8±1. 1）mL/cmH2O. The volume of the bladder in the infrasacral group was （513.2±44.37）mL; the pressure of the detrusor was （40.3±4.1）cmH2O; the compliance of the bladder（12.2±2.1）mL/cmH2O. There is a significant difference between two groups（P〈0.05）. The amounts of the mRNA of the M2 muscarinic receptors were increased in the model than the control group, there was a significant difference of the M2 receptor between the infrasacral and the suprasacral group[（1.42±0.06） vs （1.61±0.05） ,P〈0. 05]. Gonclusion The M2 muscarinic receptors are involved in the pathophysiology of the detrusor contraction in the neurogenic bladder after spinal cord injury. The M2 muscarinc receptor maybe involves the direct constraction of the infrasacral neurogenic bladder.
出处
《福建医科大学学报》
2007年第5期419-422,共4页
Journal of Fujian Medical University
关键词
膀胱
神经源性
受体
毒蕈碱
疾病模型
动物
RNA
信使
bladder, neurogenic
receptor, muscarinic
disease models, animal
RNA, messenger
