摘要
目的比较川芎、当归（不含川芎嗪）单煎和共煎液中川芎嗪含量的变化。方法采用反相高效液相色谱（RP—HPLC）法。WaterssymmetryC18色谱枉（4、6mm×150mm，5μm）；柱温25℃；流动相：水-甲醇，梯度洗脱；流速1mL·min^-1，检测波长：298nm。结果川芎嗪回归方程Y=58，4010X＋3.9318，r=0．9997，在0．25～25．00μg·mL^-1范围内相关性良好，平均加样回收率89．60％，RSD为2．7％。川芎、当归共煎液中川芎嗪含量比川芎单煎液中含量要高。结论两味中药在合煎之后川芎嗪含量明显升高，该法简便、灵敏、准确，可用于测定川芎嗪的含量。
Objective To compare the contents of tetramethylpyrazine （TMP） in the decoction of Chuanxiong rhizome alone and that of Chuanxiong rhizome together with Chinese Angelica root which does not contain TMP. Methods RP-HPLC was used in the study. The stationary phase was a Waters Symentry C18 （150 mm × 4.6 mm,5 μm）column; the column temperature was 25 ℃ ;the mobile phase was water-methanol; the flow rate was 1 mL· min^-1 in the gradient elution ;the detection wavelength was 298 nm. Results The calibration curve of TMP was linear within the concentration range of 0.25 - 25.00 μg· mL^-1,Y = 58. 4010X ＋ 3. 9318 ,r = 0.9997; The average rate of recovery was 89.60% , with anRSDof2.7%. The content of TMP in the co-decoction of the 2 drugs was significantly higher than that in the decoction of chuanxiong rhizome alone. Conclusion Co-decoction of chuanxiong rhizome and Chinese Angelica root was shown to be superior to decoction of chuanxiong rhizome alone for the extraction of TMP. The method used was handy, sensitive and accurate, and proves to be suitable for the content determination of TMP .
出处
《医药导报》
CAS
2007年第10期1207-1209,共3页
Herald of Medicine
关键词
川芎
当归
川芎嗪
色谱法
高效液相
反相
Chuanxiong rhizome
Chinese Angelica root
Tetramethylpyrazine
RP-HPLC
