摘要
目的：探讨葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏症溶血的生化机制。方法：采用荧光偏振法测定红细胞膜脂流动性；荧光分光光度法测定膜过氧化脂质（ＭＤＡ）；硅胶柱层析及薄板层析分离磷脂，气相色谱法分析磷脂中脂肪酸含量。结果：Ｇ６ＰＤ缺乏症荧光偏振度为０．２６３３±０．００４３，较正常对照的０．２２６１±０．００６９高（Ｐ＜０．０１）；Ｇ６ＰＤ缺乏时ＭＤＡ含量为１．４８２±０．０９５ｎｍｏｌ／ｍｇ膜蛋白，正常对照为０．３８２±０．０７２ｎｍｏｌ／ｍｇ膜蛋白（Ｐ＜０．０１）；Ｇ６ＰＤ缺乏症总磷脂及磷脂酰丝氨酸中不饱和脂肪酸Ｃ１８∶１，Ｃ１８∶２含量减少（Ｐ均＜０．０５）。结论：红细胞膜脂流动性降低可能是Ｇ６ＰＤ缺乏症溶血的一个重要原因。
Objective:To explore the biochemical mechanism of G6PD deficiency.Methods: A fluorescence polarization technique with 1,6 dipheny 1,1,3,5 heratciene(DPH) as a fluorescent probe was used to determine the fluidity of erythrocyte membrane lipid from G6PD deficient patients and normal controls .The polarization of fluorescence was measured between 0 0and 90 0 .Results: The fluorescence polarization parameter (ρ) was high in G6PD deficient erythrocytes as compared with normal controls,indicating a decrease in erythrocyte membrane lipid fluidity.The determination of lipoperoxidation of erythrocyte membrane by fluorescence spectrometry also showed that the content of MDA was significantly higher in G6PD deficient erythrocytes than in normal controls.The percentage of unsaturated fatty acid C 18∶1 and C 18∶2 in total phosphalipids and phosphatidyl serine was decreased in G6PD deficient erythrocytes. Conclusion:The decrease of erythrocyte membrane lipid fluidity is related to the lipid peroxidant,and the oxidative damage of erythrocyte membrane phosphalipids in G6PD deficient patients might be an important factor of hemolysis.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第4期197-199,共3页
Chinese Journal of Hematology
基金
国家自然科学基金
关键词
葡糖磷酸脱氢酶
红细胞膜
膜脂质
G6PD
Glucose 6 phosphate dehydrogenase deficiency Erythrocyte membrane Membrane lipids
