摘要
目的研究不同压强持续性CO2气腹处理对结肠癌细胞侵袭能力的影响。方法建立体外气腹模型,选用人结肠癌细胞株SW1116,分别在不同压强(6、9、12及15mmHg)99.5%医用CO2气体以及常规培养条件下暴露1h,取各压强组处理后0h和72h细胞,使用体外细胞侵袭实验模型进行体外侵袭实验。结果气腹处理后即刻(0h),15mmHg压强组结肠癌细胞侵袭荧光强度(11.36±0.861)明显低于处理前(13.43±1.113),差异有统计学意义(P=0.019),且明显低于其他各组(6mmHg组:15.58±1.015,P=0.000;9mmHg组:13.49±0.750,P=0.011;12mmHg组:13.37±0.949,P=0.016;常规培养组:13.43±2.027,P=0.014);6mmHg压强组处理后细胞侵袭荧光强度(15.58±1.015)高于处理前(14.42±1.491),但差异尚未达到统计学意义(P=0.056),且显著高于其他各压强组(9mmHg组:P=0.013;12mmHg组:P=0.009;15mmHg组:P=0.000;常规培养组:P=0.011)。气腹处理后72h,各压强组处理前、后的结肠癌细胞侵袭荧光强度差异无统计学意义(P>0.05);处理后各压强组间的结肠癌细胞侵袭荧光强度差异亦无统计学意义(P>0.05)。结论CO2气腹能对结肠癌细胞的侵袭能力产生一过性影响,随着CO2气腹压强的增高,结肠癌细胞的侵袭能力可能受到抑制。
Objective To investigate the influence of CO2-insufflation pressure on invasion potential of the colon cancer cells. Methods With an in vitro artificial pneumoperitoneum model, SWlll6 human colon cancer cells were exposed to CO2-insufflation of 5 different pressure groups： 6, 9, 12, 15 mm Hg and control group, respectively for 1 h. The invasion capacities of SWlll6 cells exposed to CO2-insufflation of 5 different pressure groups were detected by cell adhesion/invasion assay in vitro. Results Immediately following exposure to 15 mm Hg CO2 insufflation, the invasion of SWlll6 cells decreased significantly compared to the cells before exposure. At the 0 h time point, the cells exposed to 15 mm Hg were significantly less invasive than those exposed to the other insufflation pressure （P〈0.05）, and the cells exposed to 6 mm Hg were more invasive than cells exposed to the other insufflation pressure （P〈0.05）. And 72 h after exposed to CO2-insufflation, the differences between the pressure groups were not significant. Conclusion CO2-insufflation induced a temporary change in the invasion capacity of cancer cells in vitro, higher pressure of CO2-insufflation inhibits the invasion potential.
出处
《中国普外基础与临床杂志》
CAS
2007年第5期512-515,共4页
Chinese Journal of Bases and Clinics In General Surgery
关键词
结直肠肿瘤
人工气腹
细胞黏附分子
肿瘤转移
Colorectal neoplasm Artificial pneumoperitoneum Cell adhesion molecule Neoplasm metastasis
