摘要
目的:探讨前列腺相关抗原-前列腺酸性磷酸酶(PAP)在非前列腺癌中的表达水平.方法:分别用RT-PCR方法和Western Blot方法检测多种腺癌细胞系中PAP mRNA和PAP蛋白的表达水平;用免疫组织化学方法检测多种腺癌肿瘤组织中的PAP蛋白的表达水平.结果:3种结肠癌细胞(colo201,colo205和colo320)、3种胃癌细胞(MKN-7,MKN-28和MKN-45)和7种乳腺癌细胞(OCUB-F,OCUB-M,R-27,MCF-7,CRL-1500,YMB-1-E和MDA-MB-231)表达PAP mRNA和PAP蛋白,结肠癌、胃癌和乳腺癌肿瘤组织中PAP呈阳性表达.在检测的5种肾细胞癌细胞和肾细胞癌组织中均未发现有PAP的表达.结论:PAP是结肠癌、胃癌和乳腺癌的新靶点.
AIM： To study the expression of prostatic acid phosphatase （PAP） , a prostate-related antigen, in non-prostate cancer cells or tissues. METHODS： A variety of adenocareinoma cell lines were examined for their PAP expression at the mRNA and protein levels by RT-PCR and Western Blot analysis, respectively. The PAP expression in cancer tissues was also examined by immunohistoehemieal staining. RESULTS： Three kinds of colon cancer cells （colo 201, colo 205 and colo 320 ） , 3 kinds of gastric cancer cells （ MKN-7, MKN-28 and MKN-45 ）, and 7 kinds of breast cancer cell lines （ OCUB-F, OCUB-M, R- 27, MCF-7, CRL-1500, YMB-1-E and MDA-MB-231 ） as well as cancer tissues were found to be positive for PAP at both the mRNA and protein levels. But is was not found in 5 kinds of kidney cancer cells or tissues. CONCLUSION： PAP is a new target of non-prostate adenocarcinomas, including colon, breast and gastric cancers.
出处
《第四军医大学学报》
北大核心
2007年第19期1775-1777,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30600743)
关键词
酸性磷酸酶
前列腺
腺癌
acid phosphatase
prostate
adenocarcinoma
