摘要
目的:探讨胰岛素样生长因子Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)与胃癌细胞SGC7901 c-fos和c-jun表达的关系.方法:体外细胞培养,分别用MTT法和免疫组织化学以及逆转录聚合酶链反应(RT-PCR)的方法检测不同浓度IGF-Ⅱ(0,10,50,100 mg/L)作用胃癌细胞后细胞的增殖率和c-fos和c-jun蛋白及mRNA的表达情况.结果:IGF-Ⅱ作用于细胞SGC7901的增殖效应呈浓度和时间依赖性:随着药物浓度的升高,c-fos和c-jun蛋白,mRNA表达均增加.在IGF-Ⅱ10,50,100 mg/L时,c-fos和c-jun蛋白分别为41.32±1.28 mg/L,50.43±0.57 mg/L,64.22±1.76 mg/L;52.00±0.67 mg/L,63.20±0.95 mg/L,76.31±1.16 mg/L;c-fos,c-jun mRNA与GAPDH mRNA比值分别为0.316±0.021,0.392±0.0357,0.478±0.028;0.379±0.006,0.412±0.022.0.494±0.048,均与相应对照组(30.00±1.01 mg/L,41.14±2.02 mg/L,0.220±0.037,0.290±0.064)相比有统计学意义(P<0.05,P<0.01).结论:胰岛素样生长因子Ⅱ可能通过旁分泌上调c-fos和c-jun的表达而诱导胃癌细胞增殖.
AIM： To study the effects of c-fos and c-jun expression in the SGC7901 gastric cancer cell line with insulin-like growth factor （IGF）- Ⅱ.
METHODS： Proliferative activity was determined by the MTT assay, c-fos and c-jun expression was detected by immunohistochemistry and reverse transcriptase-polymerase chain reaction.
RESULTS： IGF- Ⅱ promoted the proliferation of SGC7901 human gastric cancer cells. The rate of proliferation was time and dosage dependent. Increasing concentrations of IGF-Ⅱ increased c-fos and c-jun expression at the protein and mRNA level. IGF-Ⅱ at 10, 50 or 100 mg/L resulted in c-fos and c-jun protein expression of 41.32 ± 1.28, 50.43 ± 0.57 and 64.22 ± 1.76 mg/L; and 52.00 ± 0.67, 63.20 ± 0.95 and 76.31 ± 1.16 mg/L, respectively. The ratios of c-fos and c-jun expression in mRNA level with glyceraldehyde- 3-phosphate dehydrogenase mRNA were 0.316 ± 0.021, 0.392 ± 0.0357 and 0.478 ± 0.028; and 0.379 _± 0.006, 0.412 ± 0.022, and 0.494 ± 0.048, respectively. The ratios of the experimental group were significantly different from those of the control group （30.00 ± 1.01 mg/L, 41.14 ± 2.02 mg/L, 0.220 ± 0.037, 0.290 ± 0.064, respectively）.
CONCLUSION： IGF-Ⅱ enhances the proliferation of SGC7901 cells. The possible mechanism is that IGF-Ⅱ induces gastric carcinoma cells to proliferate by increasing c-fos and c-jun expression in a paracrine manner.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第23期2477-2481,共5页
World Chinese Journal of Digestology
