摘要
目的:探讨SD大鼠孕期染镉,对胎鼠发育及心脏发生的毒性作用。方法:24只健康SD孕鼠随机分为对照组、低剂量组、中剂量组、高剂量组。在孕期第7、10、13天,对照组腹腔注射等量生理盐水,其余三组腹腔注射不同浓度的氯化镉(CdCl2)溶液。怀孕第21d观察胎鼠死胎率、心脏畸形情况;生物化学方法检测胎鼠心脏组织LDH酶活性及总蛋白含量;酶组织化学及免疫组织化学结合图像分析技术检测LDH活性的变化。结果:①高剂量组死胎率比对照组高,差异显著(P<0.01),低、中剂量组与对照组死胎率无差异(P>0.05)。活胎鼠及死胎鼠心脏,肉眼下均未见明显外观和内部分隔畸形。②中剂量组和高剂量组胎鼠心脏LDH活性比对照组显著升高(P<0.01)。③中剂量组、高剂量组胎鼠心脏总蛋白含量明显减少,与对照组差异明显(P<0.01)。结论:①孕鼠氯化镉摄入量达到一定阈值时可明显导致胚胎死亡,具有胚胎毒性,但肉眼下胎鼠心脏无明显的畸形。②氯化镉能增加胎鼠心脏LDH的活性,并存在剂量依从性。③氯化镉能抑制胎鼠心脏总蛋白的生成,对胎鼠具有心脏毒性作用。
Objective： To evaluate the cadmium toxicity to the development of fetal SD rats especially to their hearts during the pregnancy exposed to cadmium chloride of different concentration through intraperitoneal injection. Methods： Twenty-four healthy pregnant SD rats were randomly divided into the control group, the low CdCl2 treated group, the medium CdCl2 treated group and the high CdCl2 treated group. On the 7th, 10th, 13th days during the pregnant period respectively, the control group was intraperitoneally injected with the equivalent normal saline, and the other three groups were intraperitoneally injected with cadmium chloride solution of different concentration. On the 21st morning during the pregnant period, pregnant SD rats were sacrificed, The mortality rate of fetal rats and visual teratogenesis in the fetal rat hearts were observed. The LDH activities and the total protein content of the hearts in the fetal rats were examined. The changes of LDH activities were detected by enzymohistochemistry and immunohistochemistry combined with image analysis technology. Results： ①Compared with the control group, the mortality of rat embryos of the low and the medium CdCl2 treated group showed no differences （P〉0.05）, while that in the high group was significantly higher （P〈0.01）. Visual teratogenesis was not found in the hearts of both live and dead fetal rats. ②The LDH activity of the fetal heart homogenate in the medium and high dose group were significantly higher than that in the control group （P〈0.01）. ③The total protein content of the fetal heart homogenate in the medium and high dose group were significantly lower than that in the control group （P〈0.01）. Conclusions： ①Cadmium chloride has defmite embryo toxicity. When the cadmium chloride intake of the pregnant rats reached to some value, death of fetal rats would beapparently observed. Under the condition of this study, cadmium chloride had not visual teratogenic effect to the fetal rat hearts. ②Cadmium chloride can notably increase the LDH activity in a concentration-dependent manner. ③Cadmium chloride can inhibit the protein synthesis of the fetal rat hearts, thus it has definite toxicity to the fetal rat hearts.
出处
《现代生物医学进展》
CAS
2007年第10期1470-1473,1480,共5页
Progress in Modern Biomedicine
