摘要
目的观察雪旺细胞(SCs)和人胚神经干细胞(NSCs)共移植治疗帕金森病(PD)猴模型的疗效。方法采用恒河猴自体SCs和人胚NSCs共同移植治疗PD猴,3只成年恒河猴偏侧部分损伤性PD模型采用3种不同的移植物,对照猴移植磷酸缓冲液(PBS),干细胞猴移植人胚NSCs,共移植猴移植SCs+NSCs。结果猴行为学评分:在移植术后1个月,共移植猴中的各项指标有不同程度的恢复,而干细胞猴及对照猴无明显变化;2个月后,干细胞猴及共移植猴,恢复程度都在2分以上,术后4个月,共移植猴和干细胞猴已接近正常。PET示DAT的显像剂18F-FP-β-CIT在移植后2个月,共移植猴在正常侧(左侧)纹状体区呈明显的放射性浓聚,而在毁损侧(右侧)纹状体区放射性摄取也出现明显浓聚,干细胞猴毁损侧(右侧)纹状体区放射性摄取仍不明显;在移植后6个月,共移植猴和干细胞猴,DAT的显像剂在毁损侧(右侧)纹状体区放射性摄取都出现明显的浓聚,而对照猴仍无明显变化。移植区脑组织TH染色显示,对照猴中未见到TH阳性细胞,干细胞可见到有TH染色阳性细胞从移植区向周围迁移;而共移植猴可见到TH染色阳性细胞从移植区向周围迁移的数量明显增多,且细胞体积也较饱满。结论SCs和NSCs共移植能有效的治疗PD猴模型,SCs可来源于患者自体的周围神经,能避免排异反应及伦理问题,可望在临床上推广应用。
Objective To explore the effect of co-graft of Schwann cells （SCs） with neural stem cells （NSCs） on rhesus monkey with Parkinson＇ s disease （PD）. Methods Three PD rhesus monkeys were assigned to control monkey, NSCs monkey and co-graft monkey according to their grafts. PBS, NSCs, SCs plus NSCs were transplanted into the right striatum of the PD rhesus monkeys respectively. Results Monkey behavior study：One month after graft, there were significantly improvements in co-graft monkey, while there were no changes in other 2 monkeys ; Two months after graft, there were significantly improvements in co-graft monkey and NSCs monkey, while there were no changes in control monkey; Four month after graft,monkey behaviors were recovered to normal, while there were no changes in control monkey. 18F-FP-β-CIT PET study：Two months after graft, DAT binding uptake could be observed in bilateral striaturn of co-graft monkey ,while only in the left normal side of striaturn of 2 other monkeys. Four months after graft, DAT binding uptake could be observed in bilateral striaturn of co-graft monkey and NSCs monkey, while only in the left normal side of striaturn of the control monkey. Immunohistochemical staining of brain tissue：There was no TH positive cells in control monkey, while many positive ones in the NSCs monkey and the co-graft monkey were observed. The TH positive neurons in the co-graft monkey took on an appearance of plump body and multiple processes, and there was more cells contrast to the NSCs monkey. Conclusion SCs and NSCs co-graft is effective in treatment of PD rats. SCs can be exenterated easily from the peripheral nerve tissues and offer potential clinical advantages without transplant rejection due to autograft and ethical and/or legal controversies.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第9期1037-1039,共3页
Chinese Journal of Experimental Surgery
关键词
雪旺细胞
神经干细胞
帕金森病
移植
恒河猴
Schwann cells
Neural stem cells
Parkinson＇ s disease
Graft
Rhesus monkey
