摘要
使用集成化手持式SpreetaTM SPR传感器快速检测沙门氏菌,利用亲和素-生物素系统保证检测的准确性;利用沙门氏菌抗体的免疫吸附反应,保证结果的特异性;并引入复合抗体作为第二抗体以扩大检测的响应信号,检测到鼠伤寒沙门氏菌的浓度为105cfu/mL,整个检测过程在1h内完成,从一定程度实现了食品中病原微生物的快速检测。
A rapid assay for salmonella has been developed by using integrated portable SpreetaTM SPR biosensor. The assay is based on the immune adsorption of Salmonella antibody, Avidin-biotin system and secondary antibody have been used to achieve the rapid assay and guarantee the veracity, The salmonella concentration of 10^5cfu/mL can be detected in less than 1 hour.
出处
《食品科技》
CAS
北大核心
2007年第9期192-195,共4页
Food Science and Technology
基金
吉林省科技厅国际合作基金项目(20020802)
