摘要
目的:研究抗胆碱能药物盐酸苯环壬酯、盐酸去甲苯环壬酯和盐酸戊乙奎醚对映体的手性分离方法。方法:采用均匀设计方法建立了拆分3种抗胆碱能药物对映体的水介质毛细管电泳方法。也通过选择环糊精种类和浓度、背景电解质浓度和酸度等最佳试验条件,确定了非水介质毛细管电泳拆分这3种抗胆碱能药物对映体的方法。结果:40 mmol·L^(-1)的磷酸盐缓冲液,pH=2.0,含有5 mmol·L^(-1)HP-β-CD 的甲醇(20%,v/v)水溶液实现了盐酸苯环壬酯和盐酸去甲苯环壬酯的手性分离;20 mmol·L^(-1)磷酸和10 mmol·L^(-1)氢氧化钠的甲醇溶液,添加9 mmol·L^(-1)HDMS-β-CD 作为分离介质,可以同时拆分盐酸苯环壬酯和盐酸去甲苯环壬酯的混合物,并可以较好地分离盐酸戊乙奎醚对映体。结论:比较2种拆分体系表明,非水介质毛细管电泳快速、高效,分离度高。
Objective： To investigate the difference of the chiral separation of bencynonatine, demethylbencynonatine and penequinine in different capillary electrophoresis modes. Methods： Using uniform design method, bencynonatine and demethylbencynonatine were resolved by aqueous capillary electrophoresis （ACE）. For penequinine, no separation between Ⅰ and Ⅱ racemates was found, but chiral separation was obtained for enantiomer Ⅰ or Ⅱ. A simple method for the separation of 3 basic enantiomers was described by nonaqueous capillary electrophoresis （NACE） after establishing suitable separation conditions. Results：The baseline separation of 3 pairs of enantiomers was obtained using 20 mmol · L^-1phosphoric acid and 10 mmol · L^-1sodium hydroxide,containing 9 mmol· L^-1 HDMS -β -CD in methanol. Conclusion：Compared with ACE method, the NACE method provides greater efficiency, achieving baseline resolution for all 3 basic compounds in less than 23 min.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第9期1395-1398,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
水/非水介质毛细管电泳
抗胆碱能药物
手性分离
aqueous/nonaqueous capillary electrophoresis （ ACE/NACE ）
anticholinergic drug enantiomer
chiral separation
