摘要
目的：研究大孔吸附树脂分离纯化野菊花总黄酮工艺,为野菊花总黄酮的工业化生产提供实验依据。方法：以贵州产野菊花为原料,以野菊花总黄酮含量及回收率等为考察指标,选用大孔吸附树脂对野菊花总黄酮进行分离纯化,分别采用静态试验、动态试验等考察大孔树脂对野菊花总黄酮的分离纯化效果及影响因素。结果：AB-8型大孔吸附树脂对野菊花总黄酮静态饱和吸附量为114.65 mg·g^-1（干树脂）,洗脱率94.9%,动态饱和吸附量为94.5 mg·g^-1（干树脂）,总黄酮回收率在92.6%、纯度在90%以上,是实验树脂中分离纯化野菊花总黄酮的最佳大孔吸附树脂。结论：分离纯化野菊花总黄酮最佳工艺条件为：AB-8型大孔吸附树脂,洗脱剂为70%乙醇,洗脱剂用量为2-3倍树脂体积,上柱总黄酮量∶树脂=1∶10.6,上柱液总黄酮质量浓度为19.8 mg·mL^-1,流速2-3 mL·min^-1,上柱液pH 4-5。
Objective：A method for separation and purification of flavonoids from C.indicum with macroporous resin was studied.Method： By using C.indicum in Guizhou as the materials and with the content and recovery rate of flavonoids as indexes,the static and dynamic adsorption tests were employed to investigated effects and affective factors of separation and purification of flavonoids from C.indicum with macroporous resin.Result： Results show that the static adsorption capacity of AB-8 type resin was 114.65 mg·g^-1,the static elution ratio were 94.9%,the dynamic adsorption capacity of AB-8 type resin was 94.5 mg·g^-1,the recovery rate was more than 92.6% and the purity of flavonoids was more than 90%.AB-8 type resin is the best for separating and purificating C.indicum in flavonoids.Conclusion： The optimum conditions is AB-8 type macroporous resin,70% alcohol as the eluant and 2～3 times volume of the resin as the eluant volume,the ratio of flavonoids to the volume of the resin as 1∶10.6,concentration of flavonoids of sample as 19.8 mg·mL^-1 and current velocity as 2～3 mL·min^-1,pH value of sample as 4～5.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第20期2123-2127,共5页
China Journal of Chinese Materia Medica
基金
贵州省省长专项资金项目[黔省专合字(2005)261号]
贵州省"十一五"农业科技攻关项目[黔科合NY字(2006)3020]
关键词
大孔树脂
野菊花
总黄酮
分离
纯化
macroporous resin
Chrysanthemum indicum
flavonoids
separation
purification
