摘要
目的:观察复方刺五加注射液(CASI)对大鼠心肌缺血再灌注心律失常的保护作用。方法:采用在体大鼠开胸结扎冠状动脉前降支30 min后,松扎再灌注60 min造成心肌缺血再灌注模型,以心电图监测心律失常情况,并测定心肌组织超氧物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,丙二醛(MAD)及Ca2+含量变化。结果:股静脉输注CASI 25,50,100 mg.kg-1能有效防止大鼠心肌缺血再灌注室性心律失常的发生,延迟心律失常的出现时间,缩短心律失常的持续时间,降低ST段的抬高程度,可明显提高缺血再灌注心肌组织中SOD及GSH-Px活力,降低MAD及Ca2+含量。结论:CASI对大鼠心肌缺血再灌注心律失常具有明显保护作用,其机制可能与提高心肌组织的抗氧化酶活性,减少自由基及Ca2+超负荷对心肌的氧化损伤有关。
Objective：To observe the protective effect of compound acanthopanax senticosus injection（CASI） on myocardial ischemia-reperfusion arrhythmia in rats.Method： The myocardial ischemia-reperfusion model was induced by 30 min coronary occulusion and 60 min reperfusion in openchest anesthetized rats.The changes of arrhythmia with electrocardiogram lead Ⅱ,the activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px）,the contents of malondialdehyde（MDA） and Ca^2＋ in myocardium were determined.Result： In rats treated by CASI （in a dosage of 25,50 and 100 mg·kg^-1 femoral vein infusion at 30 min after coronary occulusion）,the incidence of myocardial ischemia-reperfusion ventricular arrhythmias,for instance the ventricular tachycardia（VT） and ventricular fibrillation（Vf）,was effectively prevented,the appearing time of arrhythmia was delayed and the duration of arrhythmia was shortened,while the elevated ST segment lowered as well.At the same time,the contents of myocardial Ca^2＋ and MDA were decreased significantly as well as the activities of myocardial SOD and GSH-Px increased markedly.Conclusion： CASI is of protective effect on myocardial ischemia-reperfusion arrhythmia,which may be related to scavenging the oxygen free radicals and Ca^2＋ overload formed during reperfusion.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第20期2174-2177,共4页
China Journal of Chinese Materia Medica
基金
吉林省科技厅基金项目(吉科合字第19990310-01号)
